HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Agar Medium C (Sabouraud-Glucose Agar with Antibiotics)
For selective cultivation of yeasts and moulds in accordance with European Pharmacopoeia.
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Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone (meat and casein) | 10.000 |
| Glucose monohydrate | 40.000 |
| Agar | 15.000 |
| pH after sterilization | 5.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 61.36 grams of dehydrated medium in 995 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Add aseptically rehydrated contents of one vial of Tetracycline Selective Supplement (FD196). Mix well before pouring into sterile Petri plates.
Principle And Interpretation
Sabouraud Glucose agar w/ antibiotics is cited as Medium C and recommended for cultivation of yeasts and moulds by European Pharmacopoeia (1). This medium was described originally by Sabouraud (2) for the cultivation of fungi, particularly useful for the fungi associated with skin infections. The medium is used with antibiotics such as tetracycline and benzylpenicillin (3) for the isolation of pathogenic fungi from materials containing large numbers of fungi or bacteria.
Peptone (from meat and casein) provides nitrogenous compounds. Glucose monohydrate provides an energy source. Tetracycline and benzyl penicillin inhibits a wide range of Gram-positive and Gram-negative bacteria which makes the medium selective for fungi (4). The low pH favours fungal growth and inhibits contaminating bacteria from clinical specimens (5).
Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light amber coloured clear to slightly opalescent gel forms in Petri plates
pH of 6.14% w/v aqueous solution at 25°C (after sterilization).
pH
5.40-5.80
Growth Promotion Test
Growth Promotion was carried out in accordance with the harmonized method of EP, after an incubation at 20-25°C with added Tetracycline Selective Supplement (FD196) for <=5 days.Recovery rate is considered as 100% for bacteria growth on Soybean Casein Digest Agar and fungus growth on Sabouraud Dextrose Agar
Cultural Response
| Organism | Inoculum (CFU) | Growth | Observed Lot value (CFU) | Recovery | Incubation temperature | Incubation period |
|---|---|---|---|---|---|---|
| Candida albicans ATCC 10231 | 50-100 | Luxuriant (white colonies) | 25-100 | >=50% | 20-25 °C | <=5 d |
| *Aspergillus brasiliensis ATCC 16404 | 50-100 | luxuriant | 25-100 | >=50% | 20-25 °C | <=5 d |
| Candida albicans ATCC 2091 | 50-100 | luxuriant | 25-100 | >=50% | 20-25 °C | <=5 d |
| Saccharomyces cerevisiae ATCC 9763 | 50-100 | luxuriant | 35-100 | >=50% | 20-25 °C | <=5 d |
| Escherichia coli ATCC 25922 | >=103 | inhibited | 0 | 0% | 20-25 °C | <=5 d |
| Escherichia coli ATCC 8739 | >=103 | inhibited | 0 | 0% | 20-25 °C | <=5 d |
| Escherichia coli NCTC 9002 | >=103 | inhibited | 0 | 0% | 20-25 °C | <=5 d |
| Trichophyton rubrum ATCC 28191 | 50-100 | inhibited | 0 | 0% | 20-25 °C | <=5 d |
| Lactobacillus casei ATCC 334 | >=103 | inhibited | 0 | 0% | 20-25 °C | <=5 d |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Agar Medium C (Sabouraud-Glucose Agar with Antibiotics) |
|---|---|
| SKU | ME1472 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. European Pharmacopoeia, 2008,European Department, for the Quality of Medicines |
| Customized Product Available | No |
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