HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Casein Soyabean Digest HiCynth™ Agar (Soyabean Casein Digest HiCynth™ Agar) (Tryptone Soya HiCynth™ Agar)
A general purpose medium used for cultivation of a wide variety of microorganisms from non-clinical samples and for sterility testing in pharmaceutical procedures.
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Intended use
Recommended as a general purpose medium used for cultivation of a wide variety of microorganisms.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiCynth™ Peptone No.1* | 15.000 |
| HiCynth™ Peptone No.6* | 5.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
*Chemically defined peptones
Directions
Suspend 40.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired, aseptically add 5% v/v defibrinated blood in previously cooled medium to 45-50°C for cultivation. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Soyabean Casein Digest Agar is a widely used medium, which supports the growth of wide variety of organisms even that of fastidious ones such as Neisseria, Listeria, and Brucella etc. Soyabean Casein Digest HiCynth™ Agar is a modification of Soyabean Casein Digest Agar prepared by completely replacing animal peptones with chemically defined peptones to avoid BSE/TSE risks associated with animal peptones. The medium with addition of blood provides perfectly defined haemolysis zones, while preventing the lysis of erythrocytes due to its sodium chloride content. It has been frequently used in the health industry to produce antigens, toxins etc. It's simple and inhibitor-free composition makes it suitable for the detection of antimicrobial agents in the food and other products. Soyabean Casein Digest Agar is recommended by various pharmacopoeias as sterility testing medium (1,2), and is used in microbial limit test and antimicrobial preservative - effective test. Gunn et al (3) used this medium for the growth of fastidious organisms and study of haemolytic reaction after addition of 5%v/v blood. The combination of HiCynth™ Peptone No.1 and HiCynth™ Peptone No.6 makes this media nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Sodium chloride maintains the osmotic balance. Tryptone Soya HiCynth™ Agar does not contains X and V growth factors. So it can be conveniently used in determining the requirements of these growth factors by isolates of Haemophilus by the addition of X-factor (DD020), V-factor (DD021), and X+V factor discs (DD022) factor to inoculated TSA plates (4).
Type of specimen
Pharmaceutical samples
Specimen Collection and Handling:
For Pharmaceutical samples follow appropriate techniques for sample collection, handling and processing as per pharmacopoeias (1,2). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- 1. This medium is general purpose medium and may not support the growth of fastidious organisms.
- 2. Biochemical characterization is necessary to be performed on colonies from pure cultures for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Basal Medium: Light yellow coloured clear to slightly opalescent gel. After addition of 5-7%w/v sterile defibrinated blood : Cherry red coloured opaque gel forms in Petri plates pH of 4.0% w/v aqueous solution at 25°C .
pH
7.10-7.50
Cultural response
Cultural characteristics was observed after an incubation for Bacterial at 30-35°C for 18-24 hours and for Fungal at 30-35°C <=5days.
| Organism | Inoculum (CFU) | Observed Lot value (CFU) | Recovery | Observed Lot value (CFU) w/blood | Recovery w/ blood | Haemolysis |
|---|---|---|---|---|---|---|
| Bacillus subtilis subsp. spizizenii ATCC 6633 (00003)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | none |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | beta |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | beta |
| Escherichia coli ATCC 25922 (00013)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | none |
| Escherichia coli ATCC 8739 (00012)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | none |
| Pseudomonas aeruginosa ATCC 27853 (00025)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| Pseudomonas aeruginosa ATCC 9027 (00026)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| Salmonella Abony NCTC6017(00029)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| $ Kocuria rhizophila ATCC 9341 | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| Streptococcus pneumoniae ATCC 6305 | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| Salmonella Typhimurium ATCC 14028 (00031)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| Candida albicans ATCC 10231 (00054)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| Candida albicans ATCC 2091 (00055)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
| # Aspergillus brasiliensis ATCC 16404 (00053)* | 50-100 | 25-70 | 50-70% | |||
| Clostridium sporogenes ATCC19404 (00008)* | 50-100 | 35-100 | >=70% | 35-100 | >=70% | - |
Key: (*) Corresponding WDCM numbers, (#)- Formerly known as Aspergillus niger, ($) Formerly known as Micrococcus luteus
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Casein Soyabean Digest HiCynth™ Agar (Soyabean Casein Digest HiCynth™ Agar) (Tryptone Soya HiCynth™ Agar) |
|---|---|
| SKU | MCD290 |
| Customized Product Available | No |
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