HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended use
Recommended for selective isolation of Pseudomonas species. The composition and performance of this medium are as per the specification laid down in ISO 13720:2010
Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiCynth™ Peptone No.2* | 26.000 |
| Potassium sulphate | 10.000 |
| Magnesium chloride | 1.400 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
*-Chemically Defined peptones
Directions
Suspend 52.4 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add sterile rehydrated contents of two vials of Modified CFC Selective Supplement (FD281). Mix well and pour into sterile Petri plates.
Note : Do not keep the molten agar for longer than 4 hours.
Principle And Interpretation
CFC Agar Base is prepared according to ISO (5) which contains magnesium chloride and potassium sulphate to enhance pigment production. It is recommended for enumeration of Pseudomonas species from meat and meat products including poultry by means of colony count technique after incubation at 25°C for 48 hours. Goto and Enomoto (1) formulated CetriNix supplement for the selective isolation of Pseudomonas aeruginosa from clinical specimens. Lowbury and Collins (4) studied cetrimide as a selective agent. CetriNix supplement suppresses Klebsiella, Proteus and Providencia species. Modified CFC Selective Supplement was formulated as per the recommendations of ISO (5) for selective isolation of Pseudomonas species. It contains cephalothin, sodium fusidate and cetrimide. CFC HiCynth™ Agar Base is prepared by replacing animal and vegetable peptones with chemically defined peptones to avoid BSE/TSE risks associated with animal peptones.
Type of specimen
Food samples.
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (5,6).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical tests are needed for final identification of the isolated organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Yellow coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 5.24% w/v aqueous solution at 25°C. pH : 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed with added Modified CFC Selective Supplement (FD281), after an incubation at 24-26° C for 40-48 hours.
| Organism | Inoculum (CFU) |
Growth | Recovery |
|---|---|---|---|
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant | >=50% |
| Pseudomonas fluorescens ATCC 13525 (00115*) | 50-100 | luxuriant | >=50% |
| Pseudomonas fragi ATCC 4973 (00116*) | 50-100 | luxuriant | >=50% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited | 0% |
| Proteus vulgaris ATCC 13315 | >=104 | inhibited | 0% |
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited | 0% |
| Escherichia coli ATCC 8739 (00012*) | >=104 | inhibited | 0% |
Key: (*) - Corresponding WDCM numbers
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use.
Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | CFC HiCynth™ Agar Base (Cephalothin-Sodium Fusidate - Cetrimide HiCynth™ Agar) |
|---|---|
| SKU | MCD1848 |
| Product Type | HiCynth™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1. Meat and meat products. Enumeration of presumptive Pseudomonas spp., BS EN ISO 13720:2010 |
| Customized Product Available | No |
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