HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
Recommended for primary enrichment and enumeration of Yersinia enterocolitica from food. The composition and performance criteria are in accordance with ISO 1994, ISO/DIS 10273 :2017 and ISO 11133:2014 (E) /Amd. :2020
Composition
M9411- PSB Broth, Modified
| Ingredients | g/L |
|---|---|
| Peptone # | 5.000 |
| Sorbitol | 10.000 |
| Sodium chloride | 5.000 |
| Disodium hydrogen phosphate | 8.230 |
| Sodium dihydrogen phosphate monohydrate | 1.200 |
| Bile salts | 1.500 |
| Final pH (at 25°C) | 7.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Enzymatic digest of casein
Directions
Suspend 30.77 gram (the equivalent weight of dehydrated medium per litre) in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Yersinia enterocolitica are ubiquitous, being isolated frequently from soil, water, animals, and a variety of foods. They comprise a biochemically heterogeneous group that can grow at refrigeration temperatures. PSB Broth Base is recommended by APHA (1) for enrichment of Yersinia species. PSB Broth, Modified is a modification of PSB Broth (M9411) recommended by ISO Committee (2,3). Supplementation of phosphate buffer with sorbitol and bile salts appears to be better for cold enrichment (4).
Peptone provides essential growth nutrients. Sodium chloride maintains osmotic equilibrium while phosphates buffer the medium well. Sorbitol is the energy source. Bile salts are added to make the medium selective for Yersinia species by inhibiting accompanying gram-positive bacteria.
Type of specimen
Food and Animal feeding stuff
Specimen Collection and Handling
Processing (2,3)
Enrichment: For the first initial suspension place the sample (x) in known volume of the PSB broth (M9411), to give a dilution of 1/10 dilution (by mass/volume or volume/volume). Homogenize the suspension using a peristaltic blender for 2 min. Incubate at 22°C to 25°C for 2 to 3 days with or 5 days without agitation.
For the second inition suspension in the same way with the ITC broth (M1220) so as to obtain a test portion/enrichment medium dilution of 1/100 (mass/volume or volume/volume). Incubate at 25°C for 48 hours.
Isolation:
- Inoculate the culture obtain from PSB culture on the surface of CIN agar plate and incubate at 30°C for 24 to 48 hours.
- Alkaline treatment: Using sterile pipette transfer 0.5ml of the PSB culture into 4.5 ml of KOH solution and mix for 20 seconds only. Immediately inoculate on CIN agar plate. Incubate at 30°C for 24 to 48 hours.
- Using ITC culture inoculate the surface of SSDC agar plate (M1703). Incubate at 30°C for 24 to 48 hours.
- Purification: Streak the selected colonies on the surface of Nutrient Agar (M561A). Incubate at 30°C for 24 hours.
- Confirmation: Streak the slant of the agar and incubate at 30°C for 24 hours. A black halo around the colonies obtain positive reaction.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations:
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light yellow coloured clear to very slightly hazy solution
Reaction
Reaction of 3.08% w/v aqueous solution at 25°C. pH : 7.6±0.2
pH
7.40-7.80
Cultural Response
Productivity: Cultural characteristics observed after an incubation at 25 ± 1°C for 3-5 days. Recovery is carried out on SSDC Agar (M1703)
Selectivity: Cultural characteristics observed after an incubation at 25 ± 1°C for 3-5 days Recovery is carried out on SSDC Agar (M1703)
| Organism | Inoculum (CFU) | Growth | Characteristic reaction on SSDC Agar (M1703) |
|---|---|---|---|
| Productivity | |||
| Yersinia enterocolitica subsp. enterocolitica ATCC 9610 (00038*) +Escherichia coli ATCC 8739 (00012*) +Pseudomonas aeruginosa ATCC 27853 (00025*) |
50-100 >=104 >=104 |
>10 colonies | colourless round colonies, approximately 1mm diameter. A finely granulated centre can be seen under 10X magnification |
| Yersinia enterocolitica subsp. enterocolitica ATCC 9610 (00038*) +Escherichia coli ATCC 25922 (00013*) +Pseudomonas aeruginosa ATCC 27853 (00025*) |
50-100 >=104 >=104 |
>10 colonies | colourless round colonies, approximately 1mm diameter. A finely granulated centre can be seen under 10X magnification |
| Yersinia enterocolitica biotype 1 serotype 0:8 ATCC 23715 (00160*) +Escherichia coli ATCC 8739 (00012*) +Pseudomonas aeruginosa ATCC 27853 (00025*) |
50-100 >=104 >=104 |
>10 colonies | colourless round colonies, approximately 1mm diameter. A finely granulated centre can be seen under 10X magnification |
| Yersinia enterocolitica biotype 1 serotype 0:8 ATCC 23715 (00160*) +Escherichia coli ATCC 25922 (00013*) +Pseudomonas aeruginosa ATCC 27853 (00025*) |
50-100 >=104 >=104 |
>10 colonies | colourless round colonies, approximately 1mm diameter. A finely granulated centre can be seen under 10X magnification |
| Selectivity | |||
| Pseudomonas aeruginosa ATCC 27853 (00025*) | >=104 | inhibited | |
| Proteus mirabilis ATCC 29906 (00023*) | >=104 | inhibited | |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
Reference
- Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C.
- International Organization for Standardization(ISO), 1994, Draft, ISO/DIS 10273:2017
- Microbiology of food, animal feeding stuffs and water- Preparation, production,storage and performance culture media, EN ISO 11133:2014 (E) /Amd. :2020 .
- Mehlman I.J., Aulisio C.C.G. and Sander A.C., 1978, J. Assoc. Off. Anal. Chem. 61:761.
- Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
- Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
| Product Name | PSB Broth, Modified |
|---|---|
| SKU | M941I |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Speck M. (Ed.), 1984, Compendium of Methods for The Microbiological Examination of Foods, 3rd ed., APHA, Washington,D.C. |
| Customized Product Available | No |
Write Your Own Review
PSTA Enrichment HiVeg™ Broth Base
As low as
€0.00
PSB HiVeg™ Broth Base
As low as
€0.00
Yersinia Selective Agar Base
As low as
€0.00
Cellobiose Arginine Lysine Agar (CAL Agar)
As low as
€0.00
PSB Broth Base
As low as
€0.00
Upsell Products
HiCrome™ Yersinia Agar Base
As low as
€0.00
Yersinia Selective Agar Plate
As low as
€0.00