HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Orange Serum Broth
For cultivation of microorganisms associated with spoilage of citrus products, cultivation of Lactobacilli, other aciduric organisms and pathogenic fungi.
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Intended Use
Recommended for cultivation of microorganisms associated with spoilage of citrus products, cultivation of Lactobacilli, other aciduric organisms and pathogenic fungi.
Composition
| Ingredients | g/L |
|---|---|
| Tryptone | 10.000 |
| Yeast extract | 3.000 |
| Dextrose (Glucose) | 4.000 |
| Dipotassium hydrogen phosphate | 2.500 |
| Orange serum (Solids from 200 ml) | 9.000 |
| Final pH (at 25°C) | 5.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 28.5 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense into sterile tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. AVOID OVERHEATING.
Principle And Interpretation
Fruit juices are generally acidic, with pH values ranging from approximately 2.4 for lemon juice, to 4.2 for tomato juice. The low pH of these foods is selective for yeast, moulds and a few groups of aciduric bacteria. The microorganisms of greatest significance in citrus juices are the lactic acid bacteria, primarily species of Lactobacillus and Leuconostoc, yeast and moulds. Microbial spoilage of these citrus fruit juices are most commonly due to aciduric microbes such as lactic acid bacteria and yeast. The lactic acid bacteria include Lactobacillus fermentum, L.plantarum, and Leuconostoc mesenteroides. Orange Serum Broth is recommended by APHA (1) for cultivation of Lactobacilli and other aciduric organisms. Murdock and Brokaw (2) employed Orange Serum Broth for studies of sanitary control of the processing of citrus concentrates. Hays and Reister (3) recommended Orange Serum Broth, pH 5.5 which is accepted as a control medium by the citrus industry since at this reaction, the medium is most productive for the growth of spoilage organisms. Dehydrated agar medium containing orange serum was reported by Stevens (4). Orange Serum Broth is used to initiate growth of saprophytic, pathogenic fungi in small samples (5).
Tryptone provides essential nitrogenous nutrients while dextrose serves as the fermentable carbohydrate and energy source. Yeast extract supplies B-complex vitamins, which stimulate growth. Orange serum provides an optimal environment for the recovery of acid tolerant microorganisms from citrus fruit products.
Type of specimen
Food samples - Citrus fruits
Specimen Collection and Handling:
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (1).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Medium to dark amber coloured clear solution in tubes
Reaction
Reaction of 2.85% w/v aqueous solution at 25°C. pH : 5.5±0.2
pH
5.30-5.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 40-48 hours.
| Organism | Inoculum (CFU) |
Growth |
|---|---|---|
| # Aspergillus brasiliensis ATCC 16404 (00053*) |
50-100 | good-luxuriant |
| Lactobacillus acidophilus ATCC 4356 (00098*) |
50-100 | good-luxuriant |
| Lactobacillus fermentum ATCC 9338 |
50-100 | good-luxuriant |
| Leuconostoc mesentoroides ATCC 12291 |
50-100 | good-luxuriant |
| Saccharomyces cerevisiae ATCC 9763 (00058*) |
50-100 | good-luxuriant |
| Candida albicans ATCC 10231 (00054*) |
50-100 | good-luxuriant |
Key : *Corresponding WDCM numbers.
# Formerly known as Aspergillus niger
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
References
- Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C.
- Murdock P. I. and Brokaw C. H., 1958, Food Technol., 12:573.
- MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams and Wilkins, Baltimore.
- Murdock P. I., Folinazzo J. F., and Troy V. S., 1951, Food Technol., 6:181.
- Stevens J. W., 1954, Food Technol., 8:88.
- Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
- Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
| Product Name | Orange Serum Broth |
|---|---|
| SKU | M934 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,American Public Health Association, Washington, D.C.2.Murdock P. I., Folinazzo J. F., and Troy V. S., 1951, Food Technol., 6:181.3.Stevens J. W., 1954, Food Technol., 8:88.4.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore5.Murdock P. I. and Brokaw C. H., 1958, Food Technol., 12:573. |
| Customized Product Available | No |
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