HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
Recommended for enrichment of Vibrio species.
Composition**
| Ingredients | g/L |
|---|---|
| Peptone | 5.000 |
| HM peptone B # | 3.000 |
| Sodium chloride | 30.000 |
| Bromothymol blue | 0.030 |
| Ethyl violet | 0.001 |
| Arabinose | 5.000 |
| Final pH (at 25°C) | 9.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
Suspend 43.03 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Vibrio parahaemolyticus is frequently isolated from coastal waters and seafood in temperate zones throughout the world. V.parahaemolyticus cells are often injured during food processing. Such injured cells may not be fully recovered by plating on selective media. Therefore, enrichment should involve special media to ensure the optimum recovery of both injured and healthy cells. Horie Arabinose Ethyl Violet Broth (HAE, pH 9.0) was recommended for recovering V.parahaemolyticus from refrigerated and frozen oyster homogenates (1,2,3).
Peptone and HM peptone B in the medium are sources of carbon, nitrogen, vitamins and minerals. Sodium chloride at 3.0% concentration protects cold and heat injured cells against inactivation (4). Due to fermentation of arabinose, the medium turns yellow under acidic conditions. Bromothymol blue and ethyl violet are the pH indicators.
Type of specimen
Clinical samples: faeces; Food samples
Specimen Collection and Handling:
Food sample: Weigh 50 grams of seafood sample into a blender. Add 450 ml Phosphate Buffer Saline dilution water and blend for 1 minute at 8000 rpm. This constitutes the 1:10 dilution. Prepare further dilutions and inoculate in 10 ml of Horie Arabinose Ethyl Violet Broth for enrichment. Incubate the tubes overnight at 35°C and streak a loopful from the top of the broth tubes containing the highest dilution showing growth onto TCBS Agar (M189).
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (5,6).
Warning and Precautions :
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement. Further recovery from this enriched broth onto selective media is required.
- Biochemical characterization is carried out from pure isolates for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to greenish blue homogeneous free flowing powder
Colour and Clarity of prepared medium
Blue coloured, clear solution without any precipitate
Reaction
Reaction of 4.3% w/v aqueous solution at 25°C. pH : 9.0±0.2
pH
8.80-9.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Colour of medium |
|---|---|---|---|
| Vibrio cholerae ATCC 15748 | 50-100 | good-luxuriant | yellow |
| Vibrio parahaemolyticus ATCC 17802 (00037*) | 50-100 | good-luxuriant | yellow |
Key : (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Horie Arabinose Ethyl Violet Broth |
|---|---|
| SKU | M924 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Vanderzant C. and Splittstoesser D. F., (Eds.), 1992, Compendium of Methods for the Microbiological Examination of Foods,3rd Ed., APHA, Washington, D.C.2.Wong, H. 200 |
| Customized Product Available | No |
Write Your Own Review
Alkaline Peptone Water
As low as
€0.00
Glucose Salt Teepol Broth (Twin pack)
As low as
€0.00
Upsell Products
HiCrome™ Vibrio Agar
As low as
€0.00
Alkaline Peptone Water
As low as
€0.00
Tryptone Broth w/o NaCl
As low as
€0.00
Tryptone Broth w/ 8% NaCl
As low as
€0.00
HiVibrio™ Identification Kit
As low as
€0.00