HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Recommended for enrichment and isolation of Pseudomonas species.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 7.500 |
| Tryptone | 7.500 |
| Sodium chloride | 5.000 |
| Final pH (at 25°C) | 7.2±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 20 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to room temperature and aseptically add 50 ml sterile 0.2% nitrofurantoin solution. Mix well and dispense in tubes or flasks as desired. Sterile nitrofurantoin solution (0.2%) is prepared by dissolving 1 gm Nitrofurantoin in 500 ml polyethylene glycol 300.
Note: Autosterilization takes place in 3 months. This solution can be stored for 6 months or longer.
Principle And Interpretation
Selective and differentiating media consisting of simple chemical components have been developed, both in solid and in liquid form, for culturing Pseudomonas aeruginosa. Nitrofurantoin, in the form of Macrodantin, has been shown to be active against most strains of Escherichia coli, Staphylococcus aureus and Enterococcus faecalis both in vitro and in clinical infections. Nitrofurantoin is not active against most strains of Proteus species or Serratia species. It has no activity against Pseudomonas species (3). Therefore nitrofurantoin incorporated in medium can be used as a selective medium for culturing of Pseudomonas species.
Peptone and tryptone provide the essential nutrients especially nitrogenous sources. Nitrofurantoin, 1-[(5-nitrofurfurylidene) amino] hydantoin, is a synthetic antibacterial agent which is effective against most common gram-negative and gram-positive urinary tract pathogenic bacteria (4).
Type of specimen
Dairy samples; Water samples
Specimen Collection and Handling
For dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,7).
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (2).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- 1. Due to nutritional variations and fastidious nature of organisms certain strains may show poor growth.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of Prepared medium
With added nitrofurantoin : Fluorescent yellow coloured clear solution without any precipitate
Reaction
Reaction of 2.0% w/v aqueous solution at 25°C. pH: 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | good-luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Nitrofurantoin Broth Base |
|---|---|
| SKU | M857 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Clinical and Laboratory Standards Institute, 2006, Performance standards for antimicrobial susceptibility testing. Approvedstandard M100-S15, Vol. 25, CLSI, Villanova, Pa. |
| Customized Product Available | No |
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