Asparagine Nitrate Medium is ued for the isolation and cultivation of denitrifying bacteria from soil samples. The conversion of nitrate and nitrite into molecular nitrogen or nitrous oxide through microbial processes is known as denitrification. Achromobacter denitrificans is one such organism. Asparagine is source of organic nitrogen and is readily available for microbial energy and growth while the salts in the medium help for growth of microorganisms.