HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Composition
| Ingredients | Gms/Litre |
|---|---|
| Proteose peptone | 10.000 |
| Beef extract | 1.000 |
| Sodium chloride | 5.000 |
| Mannitol | 10.000 |
| Phenol red | 0.025 |
| Agar | 15.000 |
Final pH (at 25°C): 7.4±0.2
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 41.02 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the tubed media to cool in slanted position to form slants with deep butts.
Principle And Interpretation
Phenol Red Agar media are recommended (1, 2, 3) for studying the fermentation of various carbohydrates individually by the pure cultures of microorganisms.
Proteose peptone which is free from fermentable carbohydrates is added in the medium thereby preventing the production of false positive reactions. When Phenol Red Agar with mannitol is used, a positive carbohydrate fermentation reaction is indicated by the production of a yellow colour in agar due to the effect of acid production. Gas production is indicated by the splitting of agar or by the bubbles formation. Plates or tubes may be incubated aerobically or anaerobically depending on the type of the test organism.
Quality Control
Appearance: Light yellow to pink homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Red coloured clear to slightly opalescent gel forms in tubes as slants
Reaction: Reaction of 4.1% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH: 7.20-7.60
Cultural Response
M571: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) |
Growth | Acid | Gas |
|---|---|---|---|---|
| Alcaligenes faecalis ATCC 8750 | 50-100 | luxuriant | Negative reaction,no colour change |
Negative reaction |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | Positive reaction, yellow colour |
Positive reaction |
| Klebsiella pneumoniae ATCC 13883 | 50-100 | luxuriant | Positive reaction, yellow colour |
Positive reaction |
| Proteus vulgaris ATCC 13315 | 50-100 | luxuriant | Negative reaction, no colour change |
Negative reaction |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | Positive reaction, yellow colour |
Positive reaction |
| Shigella flexneri ATCC 12022 | 50-100 | luxuriant | Positive reaction, yellow colour |
Negative reaction |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Reference
- MacFaddin J., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williams and Wilkins, Baltimore.
- Finegold and Baron, 1986, Bailey and Scotts Diagnostic Microbiology, 7th ed., The C.V. Mosby Co., St. Louis.
- Ewing, 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th ed., Elsevier Science Publishing Co., Inc., New York.
| Product Name | Phenol Red Mannitol Agar |
|---|---|
| SKU | M571 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. MacFaddin J., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williams andWilkins, Baltimore. 2.Finegold and Baron, 1986, Bailey and Scotts Diagnostic Microbiology, 7th ed., The C.V. Mosby Co., St. Louis. 3.Ewing, 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th ed., Elsevier Science Publishing Co., Inc., NewYork. |
| Customized Product Available | No |
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