ISP Medium No. 5 (Glycerol Asparagine Agar Base) is recommended for cultivation of Streptomyces species as per International Streptomyces Project. This medium provides consistent and reproducible characteristic features of Streptomyces . Glycerol serves as the carbon source while asparagine is the amino acid source for the growth of Streptomyces species. Trace mineral requirement of Streptomyces is satisfied by the trace salt solution, which contains various salts. Dipotassium phosphate buffers the medium.