Leeds Acinetobacter Agar Base

Leeds Acinetobacter Agar Base is recommended for the isolation of Acinetobacter spp. from hospital environment and for selection of MDR (Multi Drug Resistant) Acinetobacter with the addition of MDR selective supplement. Selective supplement, MDR Selective Supplement (FD271) or Leeds Acinetobacter Selective Supplement helps in inhibiting contaminating microflora. Sucrose, Fructose and Mannitol serve as the carbohydrate source. The phenylalanine serves as the substrate for enzymes which are able to deaminate it to form phenylpyruvic acid which reacts with ferric ions from ferric ammonium citrate resulting in brown black colonies by species like Providencia. The phenol red in the medium serves as a pH indicator. The acidity produced by utilization of carbohydrates results in yellow coloured colonies while the liberation of ammonia ions by the utilization of nitrogeneous material in the medium results in pink coloured colonies. Acinetobacter baumannii forms pink mucoid colonies with pink color diffused into the medium.