Sauton’s Fluid Medium Base is recommended for the cultivation and enumeration of Mycobacteria in accordance with I.P. This medium is used for determining the number of colony forming units (CFU) in vaccines of known potency as per I.P. As per the I.P. method, contents of the freeze-dried vaccine for human use are reconstituted with the diluent stated on the vial. The vaccine passes the test if 0.1 ml of the reconstituted vaccine contains between 1 x105 and 33 x 105 colony forming units.
1.Indian Pharmacopoeia, 1996, Ministry of Health and Family Welfare, Govt. of India.2.Atlas R. M., 1993, Handbook of Microbiological Media, Parks L (Ed.) CRC Press, Inc.3.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.4.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1.