HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Nutrient Agar, 1.5%
Recommended as a general purpose nutrient medium which can be used for cultivation of fastidious microorganisms after appropriate enrichment. The composition and performance criteria are in accordance with ISO 1995, ISO/DIS 13720:2010.
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Nutrient Agar 1.5% is a general purpose nutrient medium which can be used for cultivation of fastidious microorganisms after appropriate enrichment.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Beef extract | 3.000 |
| Peptic digest of animal tissue | 5.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
Final pH (at 25°C): 7.0±0.2
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 28.0 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired, it can be appropriately enriched with sterile blood, ascitic fluid or serum after cooling to 45-50°C.
Principle And Interpretation
Nutrient Agar is recommended for cultivation and maintenance of nonfastidious microorganisms. Recently ISO Committee (2) has recommended it with a slight modification (M0871) for subcultivation of Pseudomonas species isolated from meat and meat products.
Peptic digest of animal tissue is the principal source of organic nitrogen while Beef extract provides carbohydrates, vitamins, organic nitrogen compounds and salts. Sodium chloride makes the medium isotonic preventing haemolysis of red blood corpuscles. This Nutrient Agar may be used for blood culturing work after the addition of sterile 5% v/v defibrinated blood and additional Sodium chloride (3g/l) (1).
Quality Control
Appearance: Cream to yellow coloured homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Yellow coloured clear gel forms in Petri plates. With the addition of blood, cherry red coloured, opaque gel forms in petri plates.
Reaction: Reaction of 2.8% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH: 6.80-7.20
Cultural Response
M0871: Cultural characteristics observed after an incubation at 35-37°C for 18 - 24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Enterococcus faecalis ATCC 29212 | 50-100 | luxuriant | >=70% |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | >=70% |
| Pseudomonas aeruginosa ATCC 27853 | 50-100 | luxuriant | >=70% |
| Staphylococcus aureus ATCC 25923 | 50-100 | luxuriant | >=70% |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant | >=70% |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | luxuriant | >=70% |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Reference
- Speck M. (Ed.), 1984, Compendium of Methods for the Microbiological Examination of Foods, 2nd ed., APHA, Washington D.C.
- International Organization for Standardization (ISO), 1995, Draft ISO/DIS 13720.
- Pelczar, Chan and Kreig, 1986, Microbiology, 5th ed., McGraw Hill Book Co., N.Y.
| Product Name | Nutrient Agar, 1.5% |
|---|---|
| SKU | M087I |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., WashingtonD.C. 2.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination ofFoods, 5th Ed., American Public Health Association, Washington, D.C. 3.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water andWastewater, 23rd ed., APHA, Washington, D.C. 4.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition. 5.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1. 6.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C. 7.Water quality : Enumeration of Escherichia coli and total coliforms, Part 1.International Organization for Standardization (ISO),2014, Draft ISO/DIS 9308-1. |
| Customized Product Available | No |
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