HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Recommended as a selective enrichment medium for the Salmonella species, from the food & animal feeding stuffs.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Soya peptone | 4.500 |
| Sodium chloride | 7.200 |
| Dipotassium hydrogen phosphate | 0.180 |
| Potassium dihydrogen phosphate | 1.260 |
| Magnesium chloride, hexahydrate | 28.600 |
| Malachite green, oxalate | 0.036 |
| Final pH (at 25°C) | 5.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ264X bottle. Inoculate the sample and Incubate at specified temperature and time.
Principle And Interpretation
Rappaport Vassiliadis Soyabean Meal Broth (RVSM) is as per the specification laid down in ISO 6579 for the selective enrichment medium for isolation of Salmonella (3). Rappaport Vassiliadis Soyabean Meal Broth (RVSM) is modification of the Rappaport Vassiliadis Enrichment Broth, revised by van Schothorst (5,7,8). Van Schothorst modified the original formula by addition of dipotassium hydrogen phosphate to buffer the medium and addition of magnesium chloride to enhance the reliability of enrichment broth. Peterz (4) et al have also emphasized the importance of the concentration of magnesium chloride in the final medium.
The test specimen is added to Buffered Peptone Water (M1494I) and incubated at 34-36°C for 16-20 hours. This pre-enriched Buffered peptone water culture is inoculated into RVSM Broth and incubated at 41.5 ± 1°C for 24 ± 3 hours and further subcultured on XLD Agar (M0311) and Brilliant Agar w/ Phosphates (M016) or Bismuth sulphite Agar (M027). Further confirmation is carried out by isolation and biochemicals.
The medium contains soya peptone which provides essential growth nutrients. Magnesium chloride raises the osmotic pressure in the medium. Malachite green is inhibitory to organisms other than Salmonellae. The low pH of the medium, combined with the presence of malachite green and magnesium chloride, helps to select for the highly resistant Salmonella species. Phosphates buffer the medium to maintain the constant pH. Sodium chloride maintains the osmotic balance.
Type of specimen
Food, milk and millk products, animal feed, environmental samples
Specimen Collection and Handling
For Food, milk and millk products, animal feed, environmental samples samples, follow appropriate techniques for sample collection and processing as per guidelines (3,6).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidleines should be followed while handling specimens. Saftey guidelines may be referred in individual safety data sheets
Limitations
- Further subculturing on selective media is required for isolation.
Quality Control
Appearance
Sterile clear Rappaport Vassiliadis Soyabean Meal Broth in bottles.
Colour
Bluish green coloured solution.
Quantity of medium
10 ml of medium in bottles.
Reaction
5.00-5.40
Sterilization Method
Sterilized by autoclaving at 115 °C as per validated cycle
Sterility Assurance Level
Sterility assurance level of media was validated against B.subtilis Spore strips . The spore strips exposed at 115°C and unexposed strips were inoculated seperately in 100ml Soyabean Casein Digest Medium and incubated at 35°C for 7 days.
Exposed spore strips
No growth observed
Unexposed spore strips
Luxuriant growth observed
Sterility test
Passes release criteria
Cultural Response
Cultural characteristics observed after incubation at 41.5 ±1°C for 24 ± 3 hours. Recovery is carried out using XLD Agar, Modified (M0311).
| Organism | Inoculum (CFU) | Growth on XLD Agar | Recovery on XLD Agar | Colour of colony on XLD Agar |
|---|---|---|---|---|
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | good-luxuriant | >=50% | red colonies w/black centre |
| Salmonella Typhimurium ATCC 14028 (00025 *) | 50-100 | good-luxuriant | >=50% | red colonies w/black centre |
| Pseudomonas aeruginosa ATCC 27853 (00013*) | 50-100 | none-poor | <=10% | |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | none-poor | <=10% | |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | none-poor | <=10% | |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | none-poor | <=10% | |
| Enterococcus faecalis ATCC 19433 (00009*) | 50-100 | none-poor | <=10% |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 15-25°C. Use before expiry date on the label.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | Rappaport Vassiliadis Soyabean Meal Broth (RVSM Broth) |
|---|---|
| SKU | LQ264X |
| Customized Product Available | No |
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