HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use:
Recommended for cultivation of wide variety of microorganisms and for determining efficiency of sanitization of containers, equipment surfaces, water miscible cosmetics etc.
Composition**
| Ingredients | g/L |
|---|---|
| Tryptone | 17.000 |
| Soya peptone | 3.000 |
| Sodium chloride | 5.000 |
| Glucose monohydrate | 2.500 |
| Dipotassium hydrogen phosphate | 2.500 |
| Soya lecithin | 5.000 |
| Tween 20 (Polysorbate 20) | 40.000 |
| Final pH (at 25°C) | 7.3±0.5 |
**Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ193C bottle. Inoculate 50-100 cfu sample and Incubate at specified temperature and time.
Principle And Interpretation
Soybean Casein Digest Medium is recommended as a sterility testing medium in accordance with the harmonized method of USP/EP/BP/JP/IP (1-5). Soyabean Casein Digest Medium with 0.5% Soya Lecithin and 4% Tween 20 is used for the detection of microorganisms on surfaces of sanitary importance. Tryptone and soya peptone makes this medium nutritious by providing nitrogeneous and carbonaceous compounds, long chain peptides, vitamins and other essential nutrients for the growth of microorganisms. Natural sugars in soybean promote growth of fastidious organism. Sodium chloride maintains osmotic balance. Lecithin and Tween 20 are neutralizers reported to inactivate residual disinfectants from where the sample is collected (6). Lecithin neutralizes quaternary ammonium compounds and Tween 20 neutralizes preservatives in the cosmetics or pharmaceutical products, allowing bacteria to grow.
Type of specimen
Swabs of containers, Equipment surfaces, Water miscible cosmetics etc.
Specimen Collection and Handling:
For swabs of containers, equipment surfaces, water miscible cosmetics samples follow appropriate techniques for handling specimens as per established guidelines (1-5).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Warning and Precautions :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further serological and biochemical testing is required for complete identification.
Performance and Evaluation:
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature
Quality Control
Appearance: Sterile Soyabean Casein Digest Medium w/0.5% Soya Lecithin and 4%Tween 20 in glass bottle
Colour: Light yellow coloured slightly opalascent solution.
Quantity of Medium: 100 ml of medium in glass bottle.
Sterility Check: Passes release criteria
pH: 6.80-7.80
Cultural response: Cultural characteristics observed after an incubation as specified.
Growth promoting properties
Clearly visible growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating <=100 cfu at 30-35°C for 18-24 hours for bacteria and <=5 days for fungal.
| Organism | Inoculum (CFU) |
Growth | Incubation temperature |
Incubation period |
|---|---|---|---|---|
| Escherichia coli ATCC 25922(00013*) | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| ^Pseudomonas paraeruginosa ATCC 9027 (00026*) | 50 -100 | luxuriant | 30-35 °C | 18-24 hrs |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| **Bacillus spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Salmonella Abony NCTC 6017 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| $Kocuria rhizophila ATCC 9341 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | 30-35 °C | <=5 d |
| Candida albicans ATCC 2091 (00055*) | 50-100 | luxuriant | 30-35 °C | <=5 d |
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant | 30-35 °C | <=5 d |
Key: (*) Corresponding WDCM numbers,
^ Formerly known as Pseudomonas aeruginosa
# Formerly known as Aspergillus niger
** Formerly known as Bacillus subtilis subsp. spizizenii
$ Formerly known as Micrococcus luteus
Storage and Shelf Life
Store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Soyabean Casein Digest Medium w/0.5% Soya Lecithin and 4% Tween 20 |
|---|---|
| SKU | LQ193C |
| Customized Product Available | No |
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