HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended use
Recommended for the selective enrichment of Escherichia coli in accordance with harmonized methods of USP, EP, BP & JP.
Composition**
| Ingredients | g/L |
|---|---|
| Gelatin peptone # | 20.000 |
| Lactose monohydrate | 10.000 |
| Dehydrated bile ## | 5.000 |
| Bromo cresol purple | 0.010 |
| pH after sterilization (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Pancreatic digest of gelatin
## Equivalent to Dehydrated Ox-bile
Directions
Label the ready to use LQ115 bottle. Inoculate 50-100 cfu sample and Incubate at specified temperature and time.
Principle And Interpretation
MacConkey Broth is a modification of MacConkey Medium (1). Childs and Allen (2) demonstrated the inhibitory effect of neutral red and therefore substituted it by the less inhibitory bromocresol purple dye. BCP is more sensitive in recording pH variation in the medium. This medium is prepared in accordance with the harmonized method of USP/EP/BP/JP (3-6).
Gelatin peptone provides essential growth nutrients. Lactose is the fermentable carbohydrate. Dehydrated bile inhibits gram-positive organisms. Bromocresol purple is the pH indicator in the medium, which turns yellow under acidic condition. Lactose fermenting organisms turn the medium yellow due to the acidity produced on lactose fermentation. The colour change of the dye is observed when the pH of the medium falls below 6.8. Lactose non-fermenting organisms like Salmonella and Shigella do not alter the appearance of the medium.
Transfer homogenate in Soyabean Casein Digest Medium (LQ027) containing 1 gm or 1 ml of the preparation to be examined to 100 ml MacConkey Broth Incubation is carried at 43°-45°C for 24-48 hours. For further isolation, subculture on MacConkey Agar (MPH081). Growth of pink-red generally non-mucoid colonies, sometimes surrounded by a pinkish to reddish precipitation zone, indicates pressure of coliforms.
Type of specimen
Pharmaceutical samples
Specimen Collection and Handling
For pharmaceutical samples, follow appropriate techniques for sample collection, processing as per guidelines (3-6).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions:
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Though the medium is recommended for selective isolation, further biochemical and serological testing must be carried out for further confirmation.
- For further isolation, subculture on MacConkey Agar (MPH081) is required.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile clear MacConkey Broth in bottle
Colour: Purple coloured clear to slightly opalescent solution
Quantity of medium: 10ml of medium in bottle
pH: 7.10-7.50
Sterility Check: Passes release criteria
Cultural Response:
Growth Promotion is carried out in accordance with the harmonized method of USP/EP/BP/JP.
Growth promoting properties
Clearly visible growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating <=100 cfu (at 42-44°C for <= 24 hours).
Inhibitory properties
No growth of the test microorganism occurs for the specified temperature for not less than longest period of time specified inoculating >=100 cfu (at 42-44°C for >= 48 hours).
| Organism | Inoculum (CFU) |
Growth | Acid | Gas | Incubation temperature |
Incubation period |
|---|---|---|---|---|---|---|
| Growth promoting | ||||||
| Escherichia coli ATCC 8739 (00012*) |
50-100 | luxuriant | positive reaction, yellow colour | positive | 42-44 °C | <=24 hrs |
| Inhibitory | ||||||
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | >=103 | inhibited | 42-44 °C | >=48 hrs |
Key :- (*) Corresponding WDCM numbers
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | MacConkey Broth |
|---|---|
| SKU | LQ115 |
| Customized Product Available | No |
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