HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Recommended for cultivation of aerobes and anaerobes, especially pathogenic Clostridia and also for the maintenance of stock cultures.
Composition**
| Ingredients | g/L |
|---|---|
| HM granules B # | 98.000 |
| Peptone# | 20.000 |
| Sodium chloride | 5.000 |
| Glucose (Dextrose) | 5.000 |
| Yeast extract | 5.000 |
| Hemin | 0.005 |
| Vitamin K | 0.001 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
# - Equivalent to Beef extract
Directions
This product is available in multiple pack sizes of 25 and 50 bottles containing 10 ml of sterile medium in each bottle.
- The bottles when supplied are intact. Ensure that all bottles are in upright position and there is no leakage or any manufacturing defect or contamination.
- User may remove the desired number of bottles from the box as per their requirement.
- It should be handled by trained person wearing appropriate personal protective equipment (PPE) and sterile gloves.
- Place the bottles on sterile surfaces such as laminar air flow or sterile working bench.
- Label them accordingly.
- Disinfect the outer surface of cap or closures with suitable disinfectant example 70% IPA.
- Observe aseptic techniques and standard microbiological methods while processing and inoculation of samples or cultures into this media.
- On completion of inoculation tighten the cap and close it carefully.
- Incubate at specified temperature and time or as desired.
- Follow good lab practices for procedures and disposal.
Principle And Interpretation
Cooked Meat Medium w/Glucose, Hemin and Vitamin K is a modification of the Cooked Meat Medium originally developed by Robertson (1) for cultivation of anaerobes isolated from wounds. Moore et.al (2) have recommended this modified medium for subculturing of anaerobic isolates to be examined by gas liquid chromatography.
The medium contains HM granules B, which provides amino acids and nutrients. It also contains glutathione, a reducing substance which permits the growth of obligate anaerobes. The sulphydryl groups which impart reducing effect are more available in denatured protein and hence the cooked meat is added in the medium. The added supplements glucose, yeast extract, hemin and vitamin K act as growth enhancers for anaerobic microorganisms. The growth in this medium is indicated by the turbidity or bubble formation by some organisms. Blackening and disintegration of the peptone indicate proteolysis.
Type of specimen
Clinical samples: faeces, urine or other pathological materials; Food samples
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Biochemical characterization is carried out from pure isolates for complete identification.
- Recommended for Pediatric use only.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Cooked M-Medium w/Glucose, Hemin & Vitamin K in bottles.
Colour
Medium amber coloured supernatant over insoluble granules.
Quantity of medium
10ml of medium in bottles
Sterility Check
Passes release criteria
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after incubation at 35-37°C for 40-48 hours under anaerobic conditions.
| Organism | Growth (under anaerobic conditions) | Growth (under aerobic conditions) |
|---|---|---|
| Clostridium botulinum ATCC 25763 | Luxuriant | - |
| Clostridium perfringens ATCC 12924 | Luxuriant | - |
| Clostridium sporogenes ATCC 11437 | Luxuriant | - |
| Enterococcus faecalis ATCC 29212 (00087*) | - | Luxuriant |
| Streptococcus pneumoniae ATCC 6303 | - | Luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Cooked M Medium w/ Glucose, Hemin & Vitamin K |
|---|---|
| SKU | LQ093 |
| Customized Product Available | No |
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