HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended use
Recommended as an all purpose growth medium and as a base of carbohydrate fermentation media.
Composition
| Ingredients | g/L |
|---|---|
| Peptone | 10.000 |
| Sodium chloride | 5.000 |
| Final pH (at 25°C) | 7.2±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ089 bottle. Inoculate the sample and Incubate at specified temperature and time.
Principle And Interpretation
Peptone Water is particularly suitable as a substrate in the study of indole production. Peptone used in Peptone Water is rich in tryptophan content. Presence of indole can be demonstrated using either Kovacs or Ehlrich reagent. Peptone Water is also utilized as a base for carbohydrate fermentation studies with the addition of sugar and indicators such as bromocresol purple, phenol red or bromothymol blue. Peptone Water is useful for cultivation of non-fastidious organisms (1). Peptone water is formulated as per Shread Donovan and Lee (2). Peptone provides essential nutrients. Sodium chloride maintains the osmotic balance of the medium.
Type of specimen
Clinical samples: faecal dilution; Food samples; Water samples
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (3).
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (4).
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (5,6).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- 1. Further recovery from this enriched medium onto selective media is required.
- 2. Biochemical and serological characterization is required to be carried out from pure isolates for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile clear Peptone water in bottles.
Colour: Light yellow coloured medium
Quantity of medium: 5 ml of medium in bottles
pH: 7.00 -7.40
Sterility Check
Passes release criteria
Cultural response
Cultural characteristics observed after incubation at 35-37°C for 18-24 hours.
| Organism | Growth |
|---|---|
| Escherichia coli ATCC 25922 (00013*) | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | luxuriant |
| Salmonella Typhimurium ATCC 14028 (00031*) | luxuriant |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Peptone Water |
|---|---|
| SKU | LQ089 |
| Customized Product Available | No |
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