HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
For general cultivation of less fastidious microorganisms.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptic digest of animal tissue | 5.000 |
| Sodium chloride | 5.000 |
| Beef extract | 1.500 |
| Yeast extract | 1.500 |
| Final pH (at 25°C) | 7.4±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ071 bottle. Inoculate the sample and Incubate at specified temperature and time.
Principle And Interpretation
Nutrient media are basic culture media used for maintaining microorganisms, cultivating fastidious organisms by enriching with serum or blood and are also used for purity checking prior to biochemical or serological testing (1, 2). Nutrient Broth has the formula originally designed for use in the Standard Method for Examination of Water and Waste water. It is one of the several non-selective media useful in routine cultivation of microorganisms (3, 4). It can be used for the cultivation and enumeration of bacteria which are not particularly fastidious. Addition of different biological fluids such as horse or sheep blood, serum, egg yolk etc. makes it suitable for the cultivation of related fastidious organisms.
Peptic digest of animal tissue, beef extract and yeast extract provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients necessary for the growth of bacteria. Sodium chloride maintains the osmotic equilibrium of the medium.
Quality Control
Appearance: Sterile clear Nutrient Broth in bottles.
Colour: Light amber coloured medium
Quantity of medium: 5ml of medium in bottles.
Reaction: 7.20-7.60
Sterility test: Passes release criteria
Cultural response: Cultural characteristics observed after incubation at 35-37°C for 18-48 hours.
| Organism | Growth |
|---|---|
| Escherichia coli ATCC 25922 | luxuriant |
| Staphylococcus aureus ATCC 25923 | luxuriant |
| Pseudomonas aeruginosa ATCC27853 | luxuriant |
| Streptococcus pyogenes ATCC 19615 | luxuriant |
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label.
| Product Name | Nutrient Broth |
|---|---|
| SKU | LQ071 |
| Customized Product Available | No |
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