HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended use
Recommended as a qualitative test for rapid growth and confirmation of Salmonella. Combination of solid (20 ml) and liquid (40 ml) media in single bottle.
Composition**
| Ingredients | g/L |
|---|---|
| Solid | 7.000 ml |
| Peptone | 5.000 |
| HM peptone B# | 5.000 |
| Lactose | 10.000 |
| Bile salts mixture | 8.500 |
| Sodium citrate | 10.000 |
| Sodium thiosulphate | 8.500 |
| Ferric citrate | 1.000 |
| Brilliant green | 0.00033 |
| Neutral red | 0.025 |
| Agar | 15.000 |
| Liquid | 20.000 ml |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
Label the ready to use LQ029A bottle. Remove the top seal of the cap. Disinfect the part of the rubber stopper which is now exposed. Transfer the sample immediately into the culture bottle by puncturing the rubber stopper with the needle. Venting: Use sterile venting needle (LA038). Keep the bottle in an upright position preferably in a biological safety cabinet, place an alcohol swab over the rubber stopper and insert the venting needle with filter through it. Insertion and withdrawal of the needle should be done in a straight line. Discard the needle and mix the contents by gently inverting the bottle 2-3 times. Do not vent the bottle for anaerobic cultures. Incubate at 35-37°C for 18-24 hours. Recommended volume of blood to be tested in LQ029A: 8-10 ml (For Adult use).
Principle And Interpretation
SS Agar medium is recommended as differential and selective medium for the isolation of Salmonella and Shigella species from pathological specimens (1) SS Agar is a moderately selective medium in which gram-positive bacteria are inhibited by bile salts, brilliant green and sodium citrate. Peptone, HM peptone B provides nitrogen and carbon source, long chain amino acids, vitamins and essential growth nutrients. Lactose is the fermentable carbohydrate. Brilliant green, bile salts and thiosulphate selectively inhibit gram-positive and coliform organisms. Sodium thiosulphate is reduced by certain species of enteric organisms to sulphite and H2S gas and this reductive enzyme process is attributed by thiosulphate reductase. Production of H2S gas is detected as an insoluble black precipitate of ferrous sulphide, formed upon reaction of H2S with ferric ions or ferric citrate, indicated in the center of the colonies.
The high selectivity of Salmonella Shigella Agar allows the use of large inocula directly from faeces, rectal swabs or other materials suspected of containing pathogenic enteric bacilli. On fermentation of lactose by few lactose-fermenting normal intestinal flora, acid is produced which is indicated by change of colour from yellow to red by the pH indicator-neutral red. Thus these organisms grow as red pigmented colonies. Lactose non-fermenting organisms grow as translucent colourless colonies with or without black centers. Growth of Salmonella species appears as colourless colonies with black centers resulting from H2S production. Shigella species also grow as colourless colonies which do not produce H2S.
This combination is recommended for paediatric use.
Type of specimen
Clinical: blood
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (2,3).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
InVitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/ eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Recommended for adult use only.
- Further biochemical test must be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
In a sterile glass bottle combination of broth and one agar coated surface.
Colour of Agar medium
Reddish orange coloured medium
Colour of liquid medium
Red coloured medium
Quantity of medium
20ml of solid medium in glass bottle 40ml of liquid medium in glass bottle
pH of Agar medium
6.80-7.20
pH of liquid medium
6.80-7.20
Sterility test
Passes release criteria
Cultural response
Cultural characteristics was observed after incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth on agar medium | Growth on liquid medium | Colour of colony |
|---|---|---|---|---|
| Escherichia coli ATCC 25922 (00054*) | 50-100 | Fair | Fair | Pink with bile precipitate |
| # Klebsiella aerogenes ATCC 13048 (00175*) | 50-100 | Fair | Fair | Cream-Pink |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | Luxuriant | Luxuriant | Colourless colonies with black center(H2S production) |
| Salmonella Typhi ATCC 6539 | 50-100 | Luxuriant | Luxuriant | Colourless colonies with black center(H2S production) |
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | Luxuriant | Luxuriant | Colourless colonies with black center(H2S production) |
| Shigella flexneri ATCC 12022 | 50-100 | Luxuriant | Luxuriant | Colourless |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | Luxuriant | Luxuriant | Colourless |
| Salmonella Choleraesuis ATCC 12011 | 50-100 | Luxuriant | Luxuriant | Colourless colonies with black center(H2S production) |
| Proteus mirabilis ATCC 25933 | 50-100 | Luxuriant | Luxuriant | colourless, may have black centre |
Key : (*) Corresponding WDCM numbers. # formerly known as Enterobacter aerogenes
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | HiCombi™ Dual Performance Salmonella Medium - SS |
|---|---|
| SKU | LQ029A |
| Customized Product Available | No |
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