Schneider’s Insect Medium w/ Calcium chloride and Sodium bicarbonate w/o L-Glutamine

Schneider's insect medium has been specially formulated for the in vitro culture of Drosophila melanogaster cells and tissues. A number of cell lines derived from Drosophila melanogaster are now available and are extensively used in genetic and molecular biology research. Drosophila cells are also used to study recombinant protein expression. IML003 contains calcium chloride and sodium bicarbonate. It does not contain L-glutamine. The basal medium also needs to be supplemented with 5-20% of heat inactivated fetal bovine serum to supply the necessary growth factors to the insect cells. It can also be used to culture cell lines derived from other dipteran species.