Sheilds and Sang M3 Insect Medium w/ L-Glutamine w/o Potassium bicarbonate

Sheilds and Sang M3 medium is based on the original formula of D22 medium. The medium supports the growth of the cells derived from Drosophila melanogaster. Sheilds and Sang(1977) eliminated chloride salts present in the previous formulation and introduced potassium and sodium ions in the form of glutamate salts. They replaced lactabumin hydrolysate with individual amino acids and retained yeast extract. The formulation also includes Bis-Tris, an inorganic buffer which prevents shifts in pH. IM004 is Sheilds and Sangs M3 Medium with L-glutamine. It does not contain potassium bicarbonate. It needs to be supplemented with 10% fetal bovine serum. Classical Sheilds and Sangs M3(1977) medium is widely used for growth and maintenance of various Drosophila cell lines.