Hi‐PCR® Dengue Serotyping‐Chikungunya‐Zika Multiplex Probe PCR Kit

Description

Arboviral infections such as Dengue, Chikungunya and Zika are transmitted to humans through bites of infected mosquitoes specifically Aedes aegypti and Aedes albopictus. In the recent years, dengue (DENV), chikungunya (CHIKV) and Zika (ZIKV) are extending their geographical range from tropical and subtropical zones to temperate zones. Similarities in clinical manifestations and overlapping symptoms between these diseases impose a challenge in differential diagnosis. Although not very common, there are reported cases of co‐circulation of multiple DENV serotypes and co‐infection with all the three viruses in endemic regions. The above reasons underscore the importance of differential diagnosis between DENV, CHIKV and ZIKV. Hi‐PCR® Dengue Serotyping‐Chikungunya‐Zika Multiplex Probe PCR Kit allows fast, reliable solution for Dengue serotyping and detection of Chikungunya and Zika in a single assay.

NOTE: Hi‐PCR® Dengue Serotyping‐Chikungunya‐Zika Multiplex Probe PCR Kit is for in‐vitro use only.

Intended Use: Hi‐PCR® Dengue Serotyping‐Chikungunya‐Zika Multiplex Probe PCR Kit is designed for typing of Dengue virus serotypes 1‐4 and detection of Chikungunya and Zika viruses in clinical samples.

Principle

Hi‐PCR® Dengue Serotyping‐Chikungunya‐Zika Multiplex Probe PCR Kit is based on real‐time PCR technology employing reverse transcription of RNA template and subsequent pathogen specific real‐time PCR target amplification by use of hydrolysis probes that are short oligonucleotides that have a fluorescent reporter dye attached to the 5' end and a quencher dye to the 3' end. Hi‐PCR® Dengue Serotyping‐Chikungunya‐Zika Multiplex Probe PCR Kit allows simultaneous typing of Dengue serotypes DENV‐1, DENV‐2, DENV‐3 and DENV‐4 and detection of Chikungunya and Zika viruses in a two‐tube assay format. Tube 1 includes primers and probes specific for DENV‐2, DENV‐3 and DENV‐4 RNA labelled with the FAM, Texas Red and Cy5 fluorophore respectively. Tube 2 includes primers and probes specific for DENV‐1, CHIKV, ZIKV RNA labelled with the FAM, HEX and Texas Red fluorophore respectively. In addition, tube 2 contains an endogenous internal control amplification system (probe labelled with fluorophore Cy5) to verify efficient RNA isolation from clinical samples and PCR amplification.

• Simultaneous serotyping of Dengue viruses and detection of Chikungunya and Zika in a single assay
• Fast and reliable results within 90 minutes
• One‐step assay i.e. reverse transcription and amplification are performed in same tube
• Includes all reagents & controls for validity of the test
• High sensitivity and specificity
• Suitable for 4‐channel and 5‐channel qPCR cyclers
• Wet‐lab assays validated on the Bio‐Rad CFX