HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Minerals Modified Glutamate Agar Base, Granulated (Twin Pack)
Recommended for the enumeration of Escherichia coli from meat and meat products. The composition and performance criteria of this medium are as per the specifications laid down in ISO 6391:1997.
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Composition
| Ingredients | Gms / Litre |
|---|---|
| Lactose | 10.000 |
| Dipotassium phosphate | 0.900 |
| Sodium formate | 0.250 |
| L-Cystine | 0.020 |
| L-Aspartic acid | 0.024 |
| L-Arginine | 0.020 |
| Thiamine | 0.001 |
| Nicotinic acid | 0.001 |
| Pantothenic acid | 0.001 |
| Ferric ammonium citrate | 0.010 |
| Calcium chloride, 2H2O | 0.010 |
| Magnesium sulphate, 7H2O | 0.100 |
| Agar | 15.000 |
| Sodium glutamate | 6.350 |
| Final pH (at 25°C) | 6.7±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 26.29 grams (the equivalent weight of dehydrated granulated medium) of Part A and 6.35 grams of Part B in 1000 ml distilled water containing 2.5 grams ammonium chloride. Heat if necessary to dissolve the medium completely. Cool to 45-50°C. Sterilize by autoclaving at 10 lbs (115°C) for 10 minutes. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Folpmers (1) described a glutamic acid based chemically defined medium for the enumeration of coliform bacteria from water and wastewater. This glutamate-containing medium was later modified by Gray (2), by the addition of lactose, which gave less false positive results when compared to MacConkey Broth.
Mineral Modified Glutamate Agar Base is recommended for the enumeration of Escherichia coli from meat and meat products by colony count at 44°C by membrane filtration technique. Anderson and Baird-Parker (3) described a direct plate method for the rapid enumeration of E. coli in foods and this method was modified by a resuscitation procedure using Mineral Modified Glutamate Agar with successful recovery of damaged cells from frozen, dried, heat processed or low pH foods.
This media contain a variety of nutrients including salts, amino acids and vitamins. Lactose is the fermentable carbohydrate. Because of the nutrients, this media is superior for enumerating coliforms in water and wastewater as it satisfies most of the nutritional requirements of coliforms.
Quality Control
Appearance
Part A: Off-white to light yellow coloured granular medium Part B: White to cream homogenous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Yellow coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of the medium (2.634% w/v Part A + 0.635% w/v Part B + 0.25%ammonium chloride) is at 25°C. pH : 6.7±0.2
pH
6.50-6.90
Cultural Response
Cultural characteristics observed after an incubation at 44°C for 24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | >=50% |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Minerals Modified Glutamate Agar Base, Granulated (Twin Pack) |
|---|---|
| SKU | GM643I |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Folpmers T., 1948, J. Microbiol. Serol., 14.58-64 2.Gray R. D., 1959, J. Hyg. Camb. 57:249. 3.Gray R. D., 1964, J. Hyg. Camb., 62:495. 4.P. H. L. S. Standing Committee on the Bacteriological Examination of Water Supplies, 1968, J. Hyg. Camb. 65:67. 5.Abbiss J. S., Wilson J. M., Blood R. M. and Jarvis B., 1981, J. Appl. Bacteriol., 51:121. 6.Department of Environment, Health and Social Security and P. H. L. S., 1982, The Bacteriological Examination of WaterSupplies, Report on Public Health and Medical Subjects No. 71, H.M.S.O., London, England |
| Customized Product Available | No |
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