HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
YT Broth, granulated is used for the cultivation of recombinant strains of Escherichia coli.
Composition
| Ingredients | Gms/Litre |
|---|---|
| Casein enzymic hydrolysate | 16.000 |
| Yeast extract | 10.000 |
| Sodium chloride | 5.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 31.0 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
YT Broth is recommended for use in the cultivation of recombinant strains of Escherichia coli (1, 2, 3). It is also used in culture of E. coli strains for propagation of M 13 bacteriophages (2-4).
These media contain casein enzymic hydrolysate and yeast extract, which supply nitrogenous compounds, vitamin B complex and other essential nutrients and co-factors necessary for the luxuriant growth of recombinant E. coli and allows the bacteria to recover from the stress of transformation and grow well. Sodium chloride helps in maintaining isotonic conditions in the medium.
Quality Control
Appearance
Light yellow to beige coloured granular medium
Color and Clarity of Prepared Medium
Light amber coloured clear solution in tubes.
Reaction
Reaction of 3.1% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Cultural Response | ||
| Escherichia coli ATCC 23724 | 50-100 | good-luxuriant |
| Escherichia coli ATCC 53868 | 50-100 | good-luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
| Product Name | YT Broth, Granulated (2X YT Broth, Granulated) |
|---|---|
| SKU | GM1251 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Miller H., 1987, Meth. Enzymol; 152, 145. |
| Customized Product Available | No |
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