Basal Mineral Medium

Intended Use

Basal Mineral Medium is used for the cultivation of Beggiatoa species.

Composition**

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 1.53 grams in 1000 ml distilled water. Mix thoroughly. Filter sterilize. DO NOT AUTOCLAVE. Cool to 45°C and dispense into sterile test tubes.

Principle And Interpretation

Beggiatoa is most frequently found in natural environments high in heterotrophic organisms. Beggiatoa is one of the few filamentous bacteria that is motile. They have sulphur granules within its cells and attached growth is usually uncommon. Beggiatoa has a cell width of 1.0-3.0 µm and filament length of 100-500 µm. The nutritional requirements of organisms in the genus Beggiatoa are poorly understood. These organisms require dilute culture media and are inhibited by conventional media. An increase in nutrients doesnt elicit a proportional increase in cell numbers (1). Basal Mineral Medium is recommended for the cultivation of Beggiatoa species (2).This medium contains a variety of different salts in varying concentration, which provide the necessary nutrients required for the growth of Beggiatoa .

Quality Control

Appearance Cream to yellow homogeneous free flowing powder

Colour and Clarity of prepared medium Colourless clear solution without any precipitate

Cultural Response M1588: Cultural response observed after an incubation at 25-30°C, in dark,for 3-5 days.

Storage and Shelf Life

Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.

Reference

1. Lois Faust and R. S. Wolfe, J Bacteriol., 1961 January; 81(1): 99106
2. Atlas R. M., 2004, Handbook of Microbiological Media, Lawrence C.Parks (Ed.), 3rd Edition, CRC Press.