main product photo

Campylobacter Agar Base

0 Review
As low as €0.00
Availability: In stock
Only %1 left
SKU:
M994
Recommended for the selective isolation of Campylobacter species from faecal, food and environmental specimens.

Intended use

Recommended for selective isolation of Campylobacter species from faecal specimens, food and environmental specimens.

Composition**

Ingredients g/L
Proteose peptone 15.000
HL Digest# 2.500
Yeast extract 5.000
Sodium chloride 5.000
Agar 12.000
Final pH (at 25°C) 7.4±0.2

**Formula adjusted, standardized to suit performance parameters

# Equivalent to Liver digest

Directions

Suspend 19.75 grams in 500 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 40-50°C and aseptically add 5-7% v/v sterile lysed horse blood or 10% sterile defibrinated sheep blood and rehydrated contents of one vial of Blaser-Wang Selective Supplement, FD006 or Skirrow Selective Supplement, FD008. Mix well before pouring into sterile Petri plates.

Principle And Interpretation

Campylobacter species are ubiquitous in the environment inhabiting a wide variety of ecological niches (1). Infection with a Campylobacter species is one of the most common causes of human bacterial gastroenteritis (1). Most species are found in animals (cattle, swine) and cause infertility and abortion (2). C.jejuni was originally isolated on a blood-containing media with antibiotics (3). Skirrow described a selective medium for Campylobacter species consisting of Blood Agar Base No. 2 supplemented with horse blood and antibiotics (4). Subsequently, Blaser et al isolated C.jejuni on Brucella Agar supplemented with sheep blood and four antibiotics (5). Later on, a fifth antibiotic, cephalothin was added to improve the selectivity of the medium by inhibition of accompanying faecal bacteria (6). Campylobacter Agar Base is recommended by APHA for selective isolation of Campylobacter species (7).

Campylobacter Agar Base is well supplemented to support luxuriant growth of Campylobacter species. Osmotic equilibrium of the medium is maintained by sodium chloride. Blood serves as an additional source of nutrients including X-factor. The antibiotic supplements namely Blaser-Wang (FD006) and Skirrow (FD008) markedly reduce the growth of normal enteric bacteria while enhancing the growth and recovery of C.jejuni from faecal specimens. Amphotericin B in Blaser-Wang supplement greatly or completely inhibits growth of fungi. C.jejuni colonies appear non-haemolytic, flat and gray with an irregular edge or raised and round with a mucoid appearance. Some strains may appear tan or slightly pink. Swarming may be observed on moist surfaces. Incubation at 35-37°C may show a delayed growth of C.jejuni cultures. Incubating the plates at 42°C can fasten this.

The contaminated food sample (10 to 25 grams) is enriched in Campylobacter Enrichment Broth Base (M899 + FD042). The broth is incubated with agitation under a micro aerobic atmosphere for 16-18 hrs. The enrichment culture is then plated onto the selective media i.e. Campylobacter Agar Base (M994) (7).

Type of specimen

Clinical samples - faeces; Food and dairy samples; Environmental samples.

Specimen Collection and Handling:

For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (8,9).

For clinical, environmental samples follow appropriate techniques for handling specimens as per established guidelines (10,11).

After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions :

In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations :

  1. Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
  2. Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement. Well isolated colonies must be used.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance
Cream to yellow homogeneous free flowing powder

Gelling
Firm, comparable with 1.2% Agar gel.

Colour and Clarity of prepared medium
Basal medium: Yellow coloured clear gel
After addition of 5-7% v/v lysed blood: Reddish brown coloured opaque gel forms in Petri plates

Reaction
Reaction of 3.95% w/v aqueous solution at 25°C. pH: 7.4±0.2

pH
7.20-7.60

Cultural Response

Cultural characteristics observed under reduced oxygen atmosphere after an incubation at 35-37°C for 24-48 hours. (FD006-Blaser-Wang Selective Supplement/ FD008-Skirrow Selective Supplement)

Organism Growth w/
added FD006		 Growth w/
added FD008
Candida albicans ATCC 10231 (00054*) none - poor moderate
Campylobacter jejuni ATCC 29428 (00156*) good-luxuriant good-luxuriant
Escherichia coli ATCC 25922 (00013*) none - poor none - poor
Enterococcus faecalis ATCC 29212 (00087*) none - poor none - poor

Key: *Corresponding WDCM numbers.

Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (10,11).

Reference

  1. Manning H., Duim B., Wassenaar T., Wagenaar A., Ridley A., Newell D.G., 2001, Appl. Environ. Microbiol., 67:1185.
  2. Koneman E. W, Allen S. D., Janda W. M, Schreckenberger P. C., Winn W. C. Jr, 1992, Colour Atlas and Textbook of Clinical Microbiology, 4th Edition, J. B. Lippincott Company.
  3. Dekeyser P., Hossuin-Detrain M, Butzler J. P. Sterron J., 1972, J. In fect. Dis., 125: 390
  4. Skirrow M. D., 1977, Br. Med. J. 2:9
  5. Blaser M. J., Cravens B. W., Powers and Wang W. L., 1978, Lanect (ii) : 979
  6. Wilson and Wang, 1979, Information flier, Campylobacter Laboratory, Veterans Administration Hospital, Denver. Co.
  7. Vanderzant C., and Splittstoesser D. F., (Eds.), 1992, Compendium of Methods for the Microbiological Examination of foods, 3rd Ed., APHA, Washington, D.C.
  8. Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C.
  9. Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., APHA Inc., Washington, D.C.
  10. Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
  11. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
More Information
Product Name Campylobacter Agar Base
SKU M994
Product Type Regular
Physical Form Powder
Origin Animal
Packaging type HDPE
References 1. Koneman E. W, Allen S. D., Janda W. M, Schreckenberger P. C., Winn W. C. Jr, 1992, Colour Atlas and Textbook ofClinical Microbiology, 4th Edition, J. B. Lippincott Company.2.Dekeyser P., Hossuin-Detrain M, Butzler J. P. Sterron J., 1972, J. In fect. Dis., 125: 3903.Skirrow M. D., 1977, Br. Med. J. 2:94.Blaser M. J., Cravens B. W., Powers and Wang W. L., 1978, Lanect (ii) : 9795.Wilson and Wang, 1979, Information flier, Campylobacter Laboratory, Veterans Administration Hospital, Denver. Co.6.Vanderzant C., and Splittstoesser D. F., (Eds.), 1992, Compendium of Methods for the Microbiological Examination offoods, 3rd Ed., APHA, Washington, D.C.7.Manning H., Duim B., Wassenaar T., Wagenaar A., Ridley A., Newell D.G., 2001, Appl. Environ. Microbiol., 67:11858.American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., WashingtonD.C.9.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,American Public Health Association, Washington, D.C.10.Isenberg, H.D. Clinical Microbiology Procedures Handb0ook. 2nd Edition..11. Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1.12.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C.
Customized Product Available No
Write Your Own Review
You're reviewing:Campylobacter Agar Base

Related Products

Upsell Products