Bold Basal Solution (10X)

Directions :

Measure out approximately 600ml of distilled water. While stirring add 100ml of basal stock solution. Rinse the original bottle with a small volume of distilled water to remove traces of the solution. Add desired heat stable supplements prior to autoclaving. Adjust the medium to the desired pH using 1N HCl/1N NaOH. Make up the final volume to 1000ml with distilled water. Sterilize the medium by autoclaving at 15 lbs or 121ºC for 15 minutes. Cool the autoclaved medium to 45ºC before adding the filter sterilized heat labile supplements. Dispense the desired amount of medium in sterile culture vessels.

Storage and shelf life :

Store the basal stock solution at 2-8°C away from direct light. If possible, the entire content of each bottle should be used immediately aseptically after opening or else the unused portion should be stored at the given temperature. Avoid contaminating the liquid. Use before the expiry date.