HiPurA® 96 Blood Genomic DNA Purification Kit

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SKU:
MB521

HiPurA® 96 Blood Genomic DNA Purification Kit provides a fast and easy method for purification of total DNA for reliable applications in PCR, library screening and sequencing. The DNA purification procedure using the 96-well format comprises of three steps viz. adsorption of DNA to the silica gel membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedia’s 96-well format allows rapid processing of multiple samples. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, ligation, PCR and sequencing.

Recommended for isolation of DNA from blood samples. For Research Use only.

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This kit simplifies isolation of DNA from fresh and old (more than 24 hours) blood. Genomic DNA purification from blood involves cell lysis, which is achieved by incubation of whole blood in a solution containing chaotropic ions in the presence of Proteinase K at 70°C. HiMedia’s HiPurA® 96-well DNA Plate contains specially developed membranes for optimal binding of genomic DNA. After the initial binding of DNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps. High quality DNA is finally eluted in the Elution Buffer provided with the kit. Typical yield is 4-12 μg of total DNA from 200 μl of whole blood.
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Product Name HiPurA® 96 Blood Genomic DNA Purification Kit
SKU MB521
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