HiPurA® PCR Product and Gel Purification Combo Kit

The HiPurA® PCR Product and Gel Purification Combo Kit simplifies the purification of nucleic acids from enzymatic reactions viz. PCR as well as from all grades of agarose gels in a single kit. This simple procedure uses a silica-based spin column to purify DNA fragments (100bp-10kb). It is based on the advanced silica binding principle presented in a microspin format facilitating the binding, washing and elution steps thus enabling multiple samples to be processed simultaneously. DNA fragments are bound to the silica membrane, the contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt buffer or Molecular Biology Grade Water. Concentration of recovered DNA can be estimated by agarose gel electrophoresis. Typically, recoveries are upto 80% for gel extraction and 80-95% for PCR product purification from 25-100µl of PCR product and 400mg of DNA in gel.