20X TBE

20X TBE is widely used in DNA and RNA agarose gel electrophoresis and polyacrylamide gel electrophoresis. It has a higher buffering capacity than TAE buffer and for this reason TBE is preferred over TAE during DNA synthesis. TBE buffer maintains the structural integrity of nucleic acids and more suitable for their size analysis. TBE has a greater buffering capacity and will give sharper resolution than TAE buffer. In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments; whereas, TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 20X TBE is a clear liquid concentrate used to regulate the movement of negatively charged molecules through a gel matrix. This TBE Buffer functions as a standard Stock Solution for Gel Electrophoresis during any professional Molecular DNA Fragment Resolution and genotyping study.