HiPer® SGPT(ALAT) Estimation Teaching Kit is for determining the SGPT levels in serum by Reitman & Frankel method.
HiPer® SGPT(ALAT) Estimation Teaching Kit provides reagents for quantifying liver enzyme levels through kinetic or endpoint reactions. The Diagnostic Laboratory Education kit facilitates Alanine Aminotransferase Analysis and SGPT Measurement to demonstrate clinical enzyme testing principles.
HiPer® SGPT(ALAT) Estimation Teaching Kit is for determining the SGPT levels in serum. It is based of Frankel & Frankel's method. It is a colorimeteric method wherein Glutamate pyruvate transaminases GPT or Alanine transaminases (ALT) catalyses the transfer of an amino group from 1-alanine to α-ketoglutarate resulting in the formation of pyruvate and L-glutamate respectively. Pyruvate so formed couples with 2,4 DNPH in an alkaline medium to form a brown coloured hydrazone complex. The intensity of the brown colour is proportional to SGPT (ALT) activity and measured at 505nm.