Restriction enzymes are essential tools in molecular biology because each recognizes a unique DNA sequence and precisely cuts the double-stranded DNA molecule into smaller, distinct fragments. InstaDigest Pst I enzymes enables rapid digestion of DNA in 5 minutes, which helps you by saving your time to achieve best results. Pst I is a Restriction Endonuclease purified from an Providencia stuartii strain that carries the Pst I gene. The restriction enzyme Pst I recognizes the DNA sequence CTGCA^Gand optimally cleaves the DNA at 37°C when used with 10X Assay Buffer, incubating just for 5 minutes.