Restriction enzymes are essential tools in molecular biology because each recognizes a unique DNA sequence and precisely cuts the double-stranded DNA molecule into smaller, distinct fragments. InstaDigest Kpn I enzymes enables rapid digestion of DNA in 5 minutes, which helps you by saving your time to achieve best results. Kpn I is a Restriction Endonuclease purified from an Klebseilla pneumoniastrain that carries the Hind III gene gene. The restriction enzyme Kpn I recognizes the DNA sequence A^AGCTT and optimally cleaves the DNA at 37°C when used with 10X Assay Buffer, incubating just for 5 minutes.