HiPurA® XL1-Blue Competent Cells

HiPurA® XL1-Blue Competent Cells are specially prepared chemically competent cells which makes cloning experiments easier. Competent cells are ready to use bacterial cells that contain an altered cell membrane through which a foreign DNA can pass through very easily. HiPurA® XL1- Blue Competent Cells are prepared by following a modified protocol of Hanahan (1983, J.Mol.Biol 166:557) and are recommended for regular sub cloning of recombinant plasmids. XL1-Blue is the most commonly used E. coli strain for routine cloning applications through blue/white screening of colonies on plates containing X-Gal and IPTG as the lacZ∆M15 marker provides α-complementation of the β-galactosidase gene. A stock of pUC19 plasmid DNA is provided as a control to determine the transformation efficiency. To obtain maximum transformation efficiency, the experimental DNA must be free of phenol, ethanol, protein and detergents. HiPurA® XL1-Blue Competent Cells provide a high efficiency host for the uptake of foreign DNA. These Competent Cells support Blue White Screening of colonies to assist in Plasmid Transformation and any Genomic Library Construction Application.