Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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HiTouch™ Coliconfirm Agar Flexi Plate™
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$215.60
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SKU:
FL045
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HiTouch™ Flexi Plates#4fa25a
Intended use
Recommended for selective isolation and identification of E.coli and coliforms from water samples
Composition**
| Ingredients | Gms/Litre |
|---|---|
| Tryptone | 8.000 |
| Yeast extract | 0.500 |
| Growth factors | 2.20 |
| Buffers | 3.30 |
| Selective mix | 1.52 |
| Chromogenic mixture | 3.95 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Open the lid and carefully lift up the enclosed prepared medium plate so as to avoid touching the agar surface by hand. to avoid touching the agar surface by hand. Touch the surface of agar plate onto the surface to be tested. Gentlypress the plate manually for upto 10 seconds. Apply constant and uniform pressure to the whole surface. Replace exposed medium plate back in the base plate. Close the lid. Press the sides of the lid to make sure that it is fixed in the grooves. Disinfect the surface where the sample was taken in order to remove any possible traces of agar. Incubate the plates at specified temprature. After incubation as recommended count the number of colonies which have appeared on the surface of medium.
Recommended counting range for the Flexiplates is 10-100 (CFU). The number of colonies from each sample is expressed in colony forming units (CFU) extrapolated to 100 cm² of the surface sampled.
Alternative Methods of inoculation :
To use as Culture plate (ii), Sample dilution plate (iii) or Swabbing plate (iv)
The plates can be used for determination of microbial levels in liquids and fluids by dropping of 0.01 ml of sample dilution onto the medium surface.
It is also possible to inoculate this plates by moistened swab after wiping off the sample area. In case of surface with heavy contamination/ surfaces not suitable for contact plates, the swab rinse method may be used, where the swab- rinse method may be used, where the moistened swab after stroking the sample area is dipped in sterile saline and agitated. A specified amount i.e. 0.01 ml of this rinse fluid or its dilution is dropped on the surface of Flexiplates.
To use as Gravitation Settling Plate
It is also suitable for determination of airborne microorganisms in room air and otherenvironments ny gravitation settling of viable particles.
Principle And Interpretation
It is possible to remove bacteria from fluids by passing them through filters with such small pore size that bacteria are arrested. This filtration technique enables fairly large volumes of water to pass rapidly under pressure, but prevents the passage of any bacteria present. These nutrients are retained on the surface of the membrane which is then brought into contact with suitable liquid nutrients.
Escherichia coli, a member of the family Enterobacteriaceae is a normal flora of the intestinal tract of humans and a variety of animals. Although most of the E.coli does not cause gastrointestinal illness, certain groups of E.coli can cause life threatening diarrhoea and severe disability. For water testing, detection of E.coli and total coliforms is very important. There are various media available for the detection of E.coli and total coliforms.
Tryptone, growth factors provide nitrogenous and carbonaceous compound, long chain amino acids, vitamins and other essential nutrients. Phosphates buffers the medium well. Selective agents inhibit gram positive bacteria and other accompanying bacteria. The chromogenic mix incorporated in the medium aids in the detection of β-glucuronidase positive organisms which gives blue coloured organisms.
Type of specimen
Water samples.
Specimen Collection and Handling
Refer direction
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
The level of microorganisms recovery varies according to the characteristics of the surfaces and its adhesive properties (rough or smooth surfaces). HiTouch™ FlexiPlate™ is a qualitative technique for the monitoring of surface biocontamination. Taking into account the inherent inaccuracy of biological collections, it is important to use the same method of collection for all samples.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile plastic plate containing Light blue coloured firm gel
Quantity of Medium
9 ml of gel in plastic plate
Reaction
Reaction of medium is pH 7.00-7.40
Sterility Test:
Sterility of the medium was checked for 14 days. Medium was found sterile without any colony formation on surface of solid medium.
Cultural Response
Cultural characteristics was observed after incubation at 35-37° C for 18-24 hours.
Organism
| Organism | Growth | Colour of Colony |
|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | luxuriant | blue |
| Klebsiella pneumoniae ATCC 13883 (00097*) | luxuriant | maroon- red |
| # Klebsiella aerogenes ATCC 13048 (00175*) | luxuriant | maroon- red |
| Salmonella Enteritidis ATCC 13076 (00030*) | luxuriant | maroon- red |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | inhibited | - |
| Enterococccus faecalis ATCC 29212 (00087*) | inhibited | - |
| Aeromonas hydrophila ATCC 7966 | none-poor | - |
Key: (#) Formerly known as Enterobacter aerogenes, (*) corresponding WDCM numbers
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | HiTouch™ Coliconfirm Agar Flexi Plate™ |
|---|---|
| SKU | FL045 |
| Customized Product Available | No |
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