Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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HiCombi™ MacConkey-Mannitol Salt Agar Plate
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$110.00
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SKU:
HB007
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Biplates#4fa25a
Combination of MacConkey Agar + Mannitol Salt Agar recommended for cultivation and differentiation of enteric bacteria, restricting the swarming of Proteus species along with potentially pathogenic gram positive organisms especially pathogenic Staphylococci.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| MacConkey Agar | |
| Peptic digest of animal tissue | 20.000 |
| Sodium taurocholate | 5.000 |
| Gelatin peptone | 17.000 |
| Crystal violet | 0.001 |
| Beef extract | 1.000 |
| Casein enzymic hydrolysate | 1.500 |
| Peptone | 1.500 |
| Lactose | 10.000 |
| Bile salts | 0.030 |
| Neutral red | 1.500 |
| Agar | 15.000 |
| Mannitol Salt Agar | |
| Proteose peptone | 10.000 |
| Meat extract B | 1.000 |
| Phenol red | 0.025 |
| Sodium chloride | 75.000 |
| D-Mannitol | 10.000 |
| Agar | 15.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Streak the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Biplates has unique combination of two media.
MacConkey Agar is a differential medium for the selection and recovery of the Enterobacteriacae and related enteric gram-negative bacilli. This medium is prepared in accordance with USP and contain crystal violet, NaCl and Bile salts. It is very selective and suppresses growth of a number of Gram-positive bacteria including staphylococci. Coliforms and Enterobacter give pink to red colonies on this medium and Escherichia coli gives pink to red colonies with bile precipitate.
Mannitol Salt Agar is used for the selective isolation of pathogenic Staphylococci. This medium is recommended for the detection and enumeration of coagulase-positive Staphylococci in milk, food and other specimens.
Quality Control
Appearance: Sterile Mannitol Salt Agar and MacConkey Agar in 90 mm disposable biplates.
Colour: Red coloured medium with purplish tinge of MacConkey Agar and Orangish Red coloured medium of Mannitol Salt Agar
Quantity of medium: 10 ml of each medium in biplate
pH of Mannitol salt Agar: 7.20-7.60
pH of MacConkey Agar: 7.20-7.60
Sterility test: Passes release criteria
Cultural response
Cultural characteristics observed after incubation at 35-37°C for 18-48 hours.
| Organism | Growth on MacConkey Agar | Colour of colony on MacConkey Agar | Growth on Mannitol Salt Agar | Colour of colony on Mannitol Salt Agar |
|---|---|---|---|---|
| Escherichia coli ATCC 25922 | Luxuriant | Pink-red w/ bile precipitate | Inhibited | - |
| Proteus vulgaris ATCC 13315 | Luxuriant | Colourless | - | - |
| S. serotype paratyphi A ATCC 9150 | Luxuriant | Colourless | - | - |
| S. serotype paratyphi B ATCC 8759 | Luxuriant | Colourless | - | - |
| Shigella flexneri ATCC 12022 | Fair-good | Colourless | - | - |
| Staphylococcus aureus ATCC 6538 | Good-luxuriant | Pale pink - red | Luxuriant | Yellow/white surrounded by a yellow zone |
| Staphylococcus aureus ATCC 25923 | Good-luxuriant | Pale pink - red | Luxuriant | Yellow/whitesurrounded by a yellow zone |
| S. serotype enteritidis ATCC 13076 | Luxuriant | Colourless | - | - |
| Enterobacter aerogenes ATCC 13048 | Luxuriant | Pink--red | Inhibited | - |
| S. serotype typhi ATCC 6539 | Luxuriant | Colourless | - | - |
| Enterococcus faecalis ATCC 29212 | Good-luxuriant | Pale pink to red | - | - |
| Staphylococcus epidermidis ATCC 12228 | Good-luxuriant | Pale pink to red | Fair-Good | Red |
| Staphylococcus epidermidis ATCC 14990 | - | - | Fair - Good | Red |
| Proteus mirabilis ATCC 12453 | - | - | None-Poor | Yellow |
The differential action of the medium is attributed to D-Mannitol. Staphylococcus aureus ferments mannitol to produce yellow colonies with yellow cones. Most coagulase-negative species of Staphylococci and Micrococci do not ferment mannitol and therefore the medium remains red in colour. Presumptive Staphylococcus showing yellow coloured medium should be further tested for production of coagulase.
Storage and Shelf Life
Store below 20-30°C. Use before expiry date on the label.
| Product Name | HiCombi™ MacConkey-Mannitol Salt Agar Plate |
|---|---|
| SKU | HB007 |
| Customized Product Available | No |
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