main product photo

Niacin Detection Kit Modified w/o Syringe

0 Review
As low as $104.50
Availability: Out of stock
Notify me when the price drops User
Availability: In stock
Only %1 left
SKU:
K048
Download Catalogue

Composition

Ingredients Gms / Litre
Part A: Reagent (1 ml) 10.000
Part B: Reagent (1 ml) 10.000
R055: Reagent P (4 ml) 1.000

Formula adjusted, standardized to suit performance parameters

Directions

Test Sample Preparation:

  1. Use only > 3 week old Mycobacterial culture grown on Lowenstein-Jensen Medium Slants showing heavy growth. Cultures grown on other types of media do not produce enough niacin to yield a positive result by this method.

Note: False negative test results, from the use of cultures, with too few organisms or from cultures grown on media other than Lowenstein-Jensen. Do not use this test on cultures that are mixed with other Mycobacteria.

  1. Add 2 ml of sterile distilled water or saline to the slant.
  2. Cut or stab the slant with a spade or needle.
  3. Incubate the slant upright at 370C for 2 hrs.in a water bath. OR The bottle may be just kept horizontally for 20 minutes at room temperature to allow extraction. OR Place the bottle horizontally in the autoclave at 121°C for 30 minutes to allow extraction of niacin into the distilled water.
  4. Retain the slant in an upright position for 5 minutes.
  5. Use 1 ml of this solution as a test sample.

It is suggested to uselml capacity syringes for transfer of solutions.

Test:

  1. Transfer content of Part A (1ml) to Part B (1 ml). Use this as a reagent solution for further test.
  2. Transfer test sample (1 ml) to reagent solution (Part A + Part B) using a syringe.
  3. Positive reaction- Development of yellow colour within 5 minutes.
  4. Negative reaction- No development of yellow colour within 5 minutes. Reagent solution remains colourless.

Positive control :

  1. Transfer content of Part A (1ml) to Part B(1ml). Use this as a reagent solution for further test.
  2. Transfer 1 ml of R055 Reagent P to reagent solution (Part A + Part B) using syringe.
  3. Observe for development of yellow colour within 5 minutes.

Negative Control:

  1. Transfer content of Part A (1 ml) to part B (1 ml). Use this as a reagent solution for further test.
  2. Transfer 1 ml of sterile distilled water or saline into reagent solution (Part A + Part B) using syringe.
  3. Colour of solution remains colourless after 5 minutes.

Warning and Precautions

  1. The reagents being photosensitive should not be exposed to light.
  2. The reagents in Part A and B are toxic and form poisonous gas. Be careful while handling. It is advisable to cover nostrils with a face mask.
  3. Do not allow this reagent to come in contact with acid.
  4. Part A is carcinogenic. Handle with care. Do not inhale fumes or allow to come in contact with skin.

Perform all work in biological safety cabinet.

Disposal

Neutralize tubes by adding 10% NaOH to each tube before discarding.

Add 10% NaOH to each of the reagent vial and dispose by autoclaving or incineration.

Principle And Interpretation

Mycobacterium tuberculosis and some isolates of Mycobacterium simiae and Mycobacterium chelonae produce niacin during growth. These strains do not metabolize niacin further and therefore accumulate niacin which is excreted into the agar or slant. Niacin detection test kit detects the accumulated niacin and thus helps in the confirmation of M.tuberculosis .

Quality Control

Appearance

Each Kit contains Part A: Reagent (1 ml) 10 vials Part B: Reagent (1 ml) 10 vials R055 : Reagent P (4 ml) 1 vial

Colour and Clarity

Part A, Part B and R055 are clear colourless solutions free of insoluble particles.

Biochemical Test

Transfer 1 ml of R055 Reagent P to reagent solution (Part A + Part B) using syringe,Observe for development of yellow colour within 5 minutes

Biochemical Result

Yellow colour formation within 5 minutes No change in colour within 5 minutes Yellow colour formation within 5 minutes(Positive reaction)

Sterility test

Passes release criteria

Storage and Shelf Life

Store between 2-8°C. Use before expiry date on the label.

More Information
Product Name Niacin Detection Kit Modified w/o Syringe
SKU K048
Customized Product Available No
Write Your Own Review
You're reviewing:Niacin Detection Kit Modified w/o Syringe