Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Agar Granulated, Bacteriological Grade
SKU:
RM10848
CAS Number :
9002-18-0
Availability: In stock
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Agar Powder has agarobiose with same disaccharide units with α(1-3) linkage. The major source of agar is the cell wall of seaweed from Rhodophyceae family.
CAS Number :
9002-18-0
Culture Media Bases#4fa25a
Product Identifier
| Product Name | Agar Granulated |
|---|---|
| Product Code | RM10848 |
Principle And Interpretation
Agar Granulated, is recommended for culture media, disc diffusion susceptibility test where salts have been reduced to minimum. It is also suitable for electrophoretic and immunodiffusion assay including Vitamin assays and tissue culture work.
Quality Control
| Appearance | Cream coloured coarse, free flowing powder. |
|---|---|
| Solubility | Freely soluble in hot water at temperatures above 85°C. Insoluble cold water. |
| Clarity | A firm solid, clear to slightly opalescent gel is formed at a concentration of 1.5% at 34-37°C. |
| Dye Diffusion | Agar dye diffusion :- 18-20mm |
| Reaction | Reaction of 1.5% w/v aqueous solution at 25 °C |
| pH | 6.50 - 7.50 |
Identification test
| Method | As per method specified in USP 37,NF32; |
|---|---|
| A | Infrared absorption. |
| B | With Iodine, some fragments of agar appear bluish black, with some areas reddish to violet. |
| C | Agar forms a clear liquid, which congeals at 30 to 39°C to form a firm resilient gel, which does not melt below 80°C. |
Microbial Load
| Total aerobic microbial count (cfu/gm) | By plate method when incubated at 30-35°C for not less than 3 days. |
|---|---|
| Total aerobic microbial count | <= 1000 CFU/gram |
| Total Yeast and mould count (cfu/gm) | By plate method when incubated at 20-25°C for not less than 5 days. |
| Total yeast & mould Count | <= 100 CFU/gram |
Test for Pathogens
| 1. E.coli | Negative in 10 gms of sample |
|---|---|
| 2. Salmonella species | Negative in 10 gms of sample |
| 3. Pseudomonas aeruginosa | Negative in 10 gms of sample |
| 4. Staphylococcus aureus | Negative in 10 gms of sample |
| 5. C.albicans | Negative in 10 gms of sample |
| 6. Clostridia | Negative in 10 gms of sample |
Test for Water absorption
| Method | As per method specified in USP 37,NF32 |
|---|---|
| Absorption | NMT 75 ml of water is absorbed by 5.0 g of agar |
Test for Gelatin
| Method | As per method specified in USP 37,NF32 |
|---|---|
| Result | No formation of yellow precipitate |
Test for Starch
| Method | As per method specified in USP 37,NF32 |
|---|---|
| Result | No Formation of blue colour on addition of iodine |
Growth Promotion Test
| Method | As per method specified in USP 37,NF32 |
|---|
Chemical Analysis
| Gelling temperature | 34-37°C |
|---|---|
| Melting range | >=85°C |
| Water(KF) | <=20% |
| Calcium | <= 25 ppm |
| Heavy metals (as Pb) | <= 40 ppm |
| Lead | <= 10 ppm |
| Arsenic | <= 3 ppm |
| Total ash | <=6.5% |
| Acid insoluble matter (on dry basis) | <=0.5% |
| Foreign organic matter | <=1.0% |
| Foreign insoluble matter | <=15 mg in 7.5 gm of Agar |
Cultural Response
Cultural response observed after an incubation at 35-37°C for 18-24 hours by preparing Nutrient Agar (M001) using Agar Granulated as an ingredient.
| Organism | Growth |
|---|---|
| Escherichia coli ATCC 25922 | Luxuriant |
| Pseudomonas aeruginosa ATCC 27853 | Luxuriant |
| Staphylococcus aureus ATCC 25923 | Luxuriant |
| Salmonella Typhi ATCC 6539 | Luxuriant |
| Streptococcus pyogenes ATCC 19615 | Luxuriant |
Storage and Shelf Life
Store below 30°C. Use before expiry date on the label.
CAS Number :
9002-18-0
| Product Name | Agar Granulated, Bacteriological Grade |
|---|---|
| SKU | RM10848 |
| CAS No | 9002-18-0 |
| EC No. | 232-658-1 |
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