Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Fluid Casein Digest-Soya Lecithin- Polysorbate 20 Medium (Twin Pack)
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SKU:
MM117
Recommended for sanitary examination of surfaces in accordance with Indian Pharmacopoeia.
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Sanitary Examination of Surfaces and Similar Testing#CC293D
Fluid Casein Digest Soya Lecithin-Polysorbate 20 Medium is recommended for sanitary examination of surfaces in accordance with Indian Pharmacopoeia, 2007
Composition
| Ingredients | Gms/Litre |
|---|---|
| Part A | |
| Pancreatic digest of casein | 20.000 |
| Soya lecithin | 5.000 |
| Part B | |
| Polysorbate 20 | 40.000 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 25 grams of Part A in 960 ml purified/distilled water. Heat in a water bath at 48-50°C for about 30 minutes. Add 40 ml of Part B. Mix well and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Fluid Casein Digest Soya Lecithin Polysorate 20 Medium is recommended for sanitary examination of surfaces (1). Weber and Black had described the importance of a highly nutritional medium containing the neutralizing agents for quaternary ammonium compounds (2,3).
The medium contains pancreatic digest of casein which provide the necessary nutrients for the growth of the organisms. Soya lecithin neutralizes the quaternary ammonium compounds while polysorbate 20 neutralizes phenolic disinfectants, hexachlorophene and formalin (4).
This medium is also recommended by NASA for the microbiological sampling of environmental surfaces sanitized with quaternary ammonium compounds (5).
Quality Control
Appearance
Part A: Cream to yellow homogeneous free flowing powder Part B: Colourless clear viscous liquid
Colour and Clarity of prepared medium
Yellow coloured clear solution without any precipitate
Growth Promotion Test
As per Indian Pharmacopoeia.
Cultural Response
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Candida albicans ATCC 10231 | 50-100 | good-luxuriant |
| Bacillus subtilis ATCC 6633 | 50-100 | good-luxuriant |
| Escherichia coli ATCC 25922 | 50-100 | good-luxuriant |
| Escherichia coli ATCC 8739 | 50-100 | good-luxuriant |
| Staphylococcus aureus ATCC 6538 |
Storage and Shelf Life
Store below 30°C in tightly closed container and prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Fluid Casein Digest-Soya Lecithin- Polysorbate 20 Medium (Twin Pack) |
|---|---|
| SKU | MM117 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Indian Pharmacopoeia, 2007, Govt. of India, Ministry of Health and Family Welfare, Controller of Publications, New Delhi. 2.Weber and Black, 1948, Soap and Sanitary Chemicals, 24:134. 3.Weber and Black, 1948, Am. J. Public Health, 38:1405. 4.Favero (chm.), 1967, Microbiological Sampling of Surfaces, Biological Contamination Control Committee, American Asso.for Contamination Control. 5.National Aeronautics and Space Administration, 1966, Standard Procedures for the Microbiological Examination of SpaceHardware. |
| Customized Product Available | No |
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