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L-Ascorbic acid
SKU:
TC094
CAS Number :
50-81-7
Synonym :
Vitamin C
Molecular Formula :
C₆H₈O₆
Availability: In stock
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Product Identifier
| Product Code | TC094 |
|---|---|
| Molecular Weight | 176.12 |
| Molecular Formula | C₆H₈O₆ |
| CAS No. | 50-81-7 |
| Synonyms | L-Threoascorbic acid, Antiscorbutic factor, Vitamin C |
Product Description
L-ascorbic acid, also known as vitamin C is an essential micronutrient for normal growth and maintenance of cultured cells. It is used as a component of many classical and serum-free cell culture media.
Functions of L-ascorbic acid in cell culture systems:
- Promotes proliferation of some tumor cell lines as well as primary cells.
- In reactive oxygen species, it acts as a antioxidant.
- Acts as a cofactor in hydroxylation of lysine and proline residues in collagen and is essential for its normal synthesis and secretion
- In combination with other molecules such as dexamethasone, IBMX and beta-glycerophosphate, ascorbic acid regulates the differentiation of mesenchymal stem cells into osteocytes and adipocytes
- However, L-ascorbic acid is very unstable in aqueous solutions and at elevated temperatures. Its stability in culture media varies with media composition, oxygen supply, cell type and cell density.
- High doses of ascorbic acid may be detrimental to cultured cells, particularly in serum-free media. Consequently, supplementation of media with ascorbic acids should be carefully standardized.
Directions
Preparation instructions:
L-ascorbic acid solution should be sterilized by filtering through a membrane filter with porosity 0.22 microns or less. Do not autoclave or heat.
Quality Control
| Appearance | White to yellow powder. |
|---|---|
| Solubility | Clear colorless to light yellow solution at 5gm in 100ml of water. |
| pH of 5% solution in water | 2.10-2.70 |
| Specific rotation [alpha]20/D | +20.5° to +21.5° |
| Iron (Fe) | NMT 0.0002% |
| Residue on ignition | NMT 0.1% |
| Assay | NLT 99.00% |
| Cell Culture Test | Passes |
Storage and Shelf Life
| Store at | 10°-30°C. |
|---|---|
| Shelf life is | 36 months. |
| Use before | expiry date given on the product label. |
CAS Number :
50-81-7
Synonym :
Vitamin C
Molecular Formula :
C₆H₈O₆
| Product Name | L-Ascorbic acid |
|---|---|
| SKU | TC094 |
| CAS No | 50-81-7 |
| EC No. | 200-066-2 |
| Molecular formula | C₆H₈O₆ |
| Synonym | Vitamin C |
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