BES buffer, Free acid

SKU:
TC042
CAS Number : 10191-18-1
Synonym : N,N-bis[2-Hydroxyethyl]-2-aminoethanesulphonic acid
Molecular Formula : C₆H₁₅NO₅S
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Product Identifier

Product Code TC042

Product Description

Molecular Formula C₆H₁₅NO₅S
Molecular Weight 213.25
CAS No. 10191-18-1
Synonyms N,N-bis [2-Hydroxyethyl]-2-aminoethanesulfonic acid

BES buffer is a zwitterionic biological buffer used in bio-chemistry, cell culture and molecular biology. It has a pKa of 7.15 at 25°C and a useful buffering range of 6.40-7.80. BES is one of the 'Good' buffers selected by Dr Norman Good and colleagues in 1966 and has the following properties:

  • Maximum solubility in water and minimum solubility in other buffers.
  • Low contribution of ions to the medium.
  • pKa is minimally influenced by buffer concentration, temperature and ionic constitution of the medium.
  • Forms soluble complexes with metal.
  • Resists enzymatic and non-enzymatic alterations and does not react with any component of the medium.
  • Does not absorb light at wavelength longer than 230nm.
  • Is easy to prepare and easy to purify.

Technical Specification

Appearance White crystalline powder.
Solubility Clear colorless solution at 50gm in 100ml of water.
pH of 1 M solution 2.50-5.00
Heavy metal (as Pb) NMT 0.0005%
Residue on Ignition NMT 0.01%
Assay NLT 99%
Cell Culture Test Passes

Storage and Shelf Life

Store at 15-30°C.
Shelf life of the product 48 months.
Use before expiry date given on the product label.

Directions:

Preparation Instructions

BES buffer free acid is completely soluble in water at 500mg/ml.

Quality Control:

CAS Number : 10191-18-1
Synonym : N,N-bis[2-Hydroxyethyl]-2-aminoethanesulphonic acid
Molecular Formula : C₆H₁₅NO₅S
More Information
Product Name BES buffer, Free acid
SKU TC042
CAS No 10191-18-1
EC No. 233-465-5
Molecular formula C₆H₁₅NO₅S
Synonym N,N-bis[2-Hydroxyethyl]-2-aminoethanesulphonic acid
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