BES buffer is a zwitterionic biological buffer used in bio-chemistry, cell culture and molecular biology. It has a pKa of 7.15 at 25°C and a useful buffering range of 6.40-7.80. BES is one of the 'Good' buffers selected by Dr Norman Good and colleagues in 1966 and has the following properties:
Maximum solubility in water and minimum solubility in other buffers.
Low contribution of ions to the medium.
pKa is minimally influenced by buffer concentration, temperature and ionic constitution of the medium.
Forms soluble complexes with metal.
Resists enzymatic and non-enzymatic alterations and does not react with any component of the medium.
Does not absorb light at wavelength longer than 230nm.
Is easy to prepare and easy to purify.
Technical Specification
Appearance
White crystalline powder.
Solubility
Clear colorless solution at 50gm in 100ml of water.
pH of 1 M solution
2.50-5.00
Heavy metal (as Pb)
NMT 0.0005%
Residue on Ignition
NMT 0.01%
Assay
NLT 99%
Cell Culture Test
Passes
Storage and Shelf Life
Store at
15-30°C.
Shelf life of the product
48 months.
Use before
expiry date given on the product label.
Directions:
Preparation Instructions
BES buffer free acid is completely soluble in water at 500mg/ml.