Schneider’s Insect Medium w/ L-Glutamine w/o Calcium chloride and Sodium bicarbonate

Schneider's insect medium has been specially formulated for the in vitro culture of Drosophila melanogaster cells and tissues. A number of cell lines derived from Drosophila melanogaster are now available and are extensively used in genetic and molecular biology research. IM003 contains L-glutamine. It does not contain calcium chloride and sodium bicarbonate, hence needs to be supplemented with calcium chloride and sodium bicarbonate while preparing the complete medium. The basal medium also needs to be supplemented with 5-20% of heat inactivated fetal bovine serum to supply the necessary growth factors to the insect cells. It can also be used to culture cell lines derived from other dipteran species.