Dulbecco's Modified Eagle Medium (DMEM), High glucose w/ 4.5gms Glucose per litre, 25mM HEPES buffer and Sodium bicarbonate w/o L-Glutamine and Sodium pyruvate

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells. DMEM high glucose is a further modification of original DMEM and contains 4500mgs/L of glucose. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines. AL067 is DMEM with 4.5gms per litre glucose, 25mM HEPES buffer and sodium bicarbonate. It does not contain L-glutamine and sodium pyruvate. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium.