ITS-E Supplement 100X, Liquid w/ 1 mg per ml Insulin from bovine pancreas, 0.55 mg per ml Human Transferrin, 0.5 µg per ml Sodium Selenite and 0.2 mg per ml Ethanolamine in Earle’s Balanced Salt Solution w/o Phenol red

ITS-E supplement is a basal growth factor supplement used to reduce the serum requirements in reduced serum and serum free cell culture systems. Insulin is a peptide hormone responsible for uptake, utilization and storage of glucose by cells. Insulin is often used as a medium supplement for cell culture in the range of 1-10µg/ml depending upon the cell type. Transferrin is a glycoprotein responsible for delivery of iron to cells. Most of the stimulatory activity of transferrin is attributed to its own iron binding properties. Transferrin has a stimulatory effect on many cell types and is used in cell culture at a concentration of 1-10µg/ ml. Selenium is an essential trace element required for normal cell growth and development. Ethanolamine is a fatty acid that is frequently used as a supplement to promote growth of hybridoma cells.