1M Sulphuric Acid

Availability: Out of stock
Availability: Out of stock
Only %1 left
SKU:
ML187
Sulphuric acid plays a major role in ELISA experiments as a Stop solution where 3,3’,5,5’ - tetramethylbenzidine (TMB) acts as a substrate.


Sulphuric acid plays a major role in ELISA experiments as a Stop solution where 3,3’,5,5’ - tetramethylbenzidine (TMB) acts as a substrate. Stop solution should fulfil two major requirements: 1) to terminate the reaction by effectively inhibiting the enzymatic activity of HRP, 2) to stabilize the oxidized products of the chromogenic or fluorogenic substrates. In the case of TMB, the stop solution, Sulphuric acid, not only inhibits color development but also converts the blue oxidation product of TMB to a yellow derivative which has an absorbance maxima at 450mn.
More Information
Product Name 1M Sulphuric Acid
SKU ML187
Customized Product Available No
Write Your Own Review
You're reviewing:1M Sulphuric Acid
Your Rating