5X Tris-Glycine-SDS Gel Running Buffer is the most commonly used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE). This buffer is used as both anode and cathode buffer. This buffer is supplied as 5X concentrated stock solution and should be diluted to 1X with deionized water before usage.
5X Tris-Glycine-SDS Gel Running Buffer composed of 0.5 M Tris, 1.92 M Glycine, 0.5% SDS is the most commonly used as the electrophoresis
buffer during the stacking and resolve process of sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE). This buffer is used as both anode and cathode buffer