5X Tris-SDS Buffer (pH 6.8)

Product Identifier

Introduction

5X Tris-SDS Buffer (pH 6.8) is used to prepare buffer for stacking gel during SDS-PAGE (SDS-Polyacrylamide gel electrophoresis). It forms discontinuous stacking gel with a large pore size region for concentrating protein sample before entering separating zone. This buffer is supplied as a 5X stock.

Description

5X Tris-SDS Buffer (pH 6.8) is used in the preparation of stacking gel mix while performing SDS-PAGE. It is used for the separation of proteins through electrophoresis and it is based on the fact that charged molecules will migrate through a matrix upon application of an electrical field. The matrix for protein electrophoresis separation is polyacrylamide. SDS-PAGE uses two types of buffer systems: the continuous buffer system and the discontinuous buffer system. In the continuous buffer system the pH of the gel matrix remains constant throughout the separation. In contrast, the discontinuous buffer system consists of a narrow layer of stacking gel (of large pore size and acidic pH, 6.8) above the main separating gel matrix of alkaline pH. The stacking gel contains chloride ions, which migrate more quickly through the gel than the protein sample, while the electrophoresis buffer contains glycine ions, which migrate more slowly. The protein molecules are trapped in a sharp band between these ions. The stacking gel concentrates the protein sample before entering the separating gel which enhances the resolution. SDS-PAGE with a discontinuous buffer system is the most popular electrophoresis technique used to analyze polypeptides.

Application

The Tris-SDS Buffer (pH 6.8) solution is used in protein and nucleic acid electrophoresis. The solutions required for preparation of a 10 ml resolving gel for Tris-Glycine-SDS-PAGE are tabulated as follows:

Storage Conditions

The 5X Tris-SDS Buffer, pH 6.8 has to be stored at 2-8° C. Under recommended condition, the reagent is stable for 12 months.

Properties

Warning and Precautions

Not for Medicinal Use. Read the SDS carefully before beginning the protocol. Wear protective gloves/protective clothing/eye protection/face protection. Follow good clinical laboratory practices while handling clinical samples. Standard precautions should be followed as per established guidelines. Safety guidelines may be referred in safety data sheets of the product.

Performance and Evaluation

Performance of the solution is expected when the solution is stored at recommended temperature and within the expiry period.

Safety Information

The 5X Tris-SDS Buffer (pH 6.8) is for laboratory use only, not for drug, household or other uses. Take appropriate laboratory safety measures and wear gloves and safety goggles when handling. Not compatible with disinfecting agents containing bleach. Please refer the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices

Technical Assistance

At HiMedia we pride ourselves on the quality and availability of our technical support. For any kind of technical assistance, mail at mb@himedialabs.com.