20X SSC buffer has a high ionic strength as it contains Sodium chloride and it utilizes citrate as a buffering system. This solution is formulated for use in nucleic acid hybridizations and blot transfer applications in concentrations ranging from 0.2X to 20X, depending upon the application
20X SSC buffer has a high ionic strength as it contains Sodium chloride and it utilizes citrate as the buffering system. This buffer is used in DNA and RNA probecontaining hybridization cocktails and to control stringency for washing steps of Southern and Northern blotting.