Hi-SYBr Master Mix (with Hi-Temp DNA Polymerase), supplied in 2X concentration, is a ready-to-use mix,
convenient for Real-Time PCR. The master mix contains:
1.SYBr Green Dye
2.Hi-Temp DNA polymerase
3.dNTPs
4.Assay buffer
5.MgCl2
Hi-SYBr Master Mix contains Hi-Temp DNA Polymerase for hot-start PCR, to improve specificity and sensitivity of the PCR by minimizing the formation of non-specific amplification products. The enzyme is active only at high temperatures, where primers no longer bind non-specifically.